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CD47 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666948

Human CD47 (IAP, Integrin Associated Protein) is a broadly expressed Ig superfamily glycoprotein of about 50kD with a long extracellular Ig like domain, 5 transmembrane domains and an alternatively spliced intracellular c-terminal domain. It interacts with it… More details

$435 / 120 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD47, Monoclonal Antibody
Full Product Name
Monoclonal anti-human CD47/FITC
Product Gene Name
Product Synonym Names
anti-CD47; anti-human CD47; IAP; Integrin Associated Protein
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
ANC2F6
Host
Mouse
Species Reactivity
Human
Form/Format
50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).
Concentration
Volume in Vial: 0.2 ml (lot specific)
Specificity
Clone ANC2F6 binds to an epitope within the extracellular Ig-like domain of human CD47.
Preparation and Storage
Store at 2-5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Immunogen
Recombinant human CD47 (extracellular Ig domain)
Conjugation
FITC
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using adesalting column. Antibody conjugate is at 0.5mg/ml with a fluorescein: antibody molar ratio of 7.1.
Performance
Five x 105 cultured CEM cells were washed and incubated 45 minutes on ice with 80ul of antiCD47/FITC at a dilution factor of 1:50 (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cellsstained positive with a mean shift of 2.04 log10 fluorescent units when compared to a Mouse IgG1/FITC isotype control (#MBS666714). Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD47antibody (#MBS666928).
Applications Tested/Suitable for anti-CD47 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CD47 antibody
Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Testing Data of anti-CD47 antibody

Mouse Anti-CD47 Monoclonal Antibody – Cat# MBS666948

Product References and Citations for anti-CD47 antibody

1) Xiaojuan Liu, Yang-xin Fu, et al. (2017) J Hematol Oncol 10:12. doi:10.1186/s13045-016-0381-z PMID: 28077173.
2) Wu L, Sun ZJ, et al. (2018) Oncoimmunology 7 (4): e1397248. Doi: 10.1080/2162402X.2017.1397248.
3) Lif F, Jiang X, et al. (2017) Oncoimmunology 7 (2): e1391973. doi: 10.1080/2162402X.2017.1391973.
4) Norman MZ, Janji B, et al. (2018) Oncoimmunology. 7 (4):e1345415. doi: 10.1080/2162402X.2017.1345415.

Other Notes

Small volumes of anti-CD47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD47 purchase

MBS666948 is an antibody targeting CD47. Also known as Monoclonal anti-human CD47/FITC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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