Mouse Anti-CD155 Monoclonal Antibody – Cat# MBS666954

CD155 Antibody – Mouse Monoclonal

Name: CD155 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666954
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666954

Human CD155 (Polio Virus Receptor, PVR, Necl-5) is a 70kd type I Ig superfamily molecule (1).1 It is involved in formation of intracellular junctions between epithelial cells. Its ligands include CD226 (DNAM-1), and CD96 (TACTILE). CD155 expression by tumor… More details

$675 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD155, Monoclonal Antibody

Full Product Name

Monoclonal anti-human CD155 (PVR)/R-PE

Product Gene Name

anti-CD155 antibody

Product Synonym Names

anti-CD155 (PVR, ANC2B2); anti-human CD155 (PVR); Poliovirus Recdeptor; PVR; Necl-5

Clonality

Monoclonal

Isotype

Mouse IgG1 kappa

Clone Number

ANC2B2

Host

Mouse

Species Reactivity

Human

Form/Format

50mM Sodium Phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).

Concentration

Volume in Vial: 0.2 ml (lot specific)

Specificity

Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.
Antibodies ANC2B2 and ANC6A3 (#MBS666907) each bind to distict epitopes of CD155 and are suitable as a matched pair for sandwich EIA.

Preparation and Storage

Store at 2-5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Conjugation

R-PE

Production

Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrinthrough a sulfo-ester linkage. Unconjugated antibody wasremoved using size exclusion chromatography. Antibody conjugate is at 1.0mg/ml with an A565/A280 ratio of 3.04.

Performance

Five x 105 cultured human U-937 cells pertube were washed and preincubated 5 minutes with 20ul of 300ug/ml human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-human CD155/R-PE at a dilution factor of 1:50 (20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stainedpositive with a mean shift of 0.5 log10 fluorescent units whencompared to a Mouse IgG1/R-PE negative control (#MBS666704) at a similar concentration. Binding was blocked when cellswere preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD3antibody (#MBS666567).

Applications Tested/Suitable for anti-CD155 antibody

ELISA (EIA), Flow Cytometry (FC/FACS)

Application Notes for anti-CD155 antibody

Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD155 antibody

1) Medelsohn CL, Racaniello VR, et al. (1989) Cell 56 (5): 855-65.
2) C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15 (1):17 PMID 25609078.
3) Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16 (supp3): iii41.
4) Baury B, MG Denis, et al. (2003) Biochem Biophys Res Comm 309: 175-82.

Other Notes

Small volumes of anti-CD155 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD155 purchase

MBS666954 is an antibody targeting CD155. Also known as Monoclonal anti-human CD155 (PVR)/R-PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD155. It may not necessarily be applicable to this product.

Protein Family

Poliovirus receptor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.