Mouse Anti-CD252 Monoclonal Antibody – Cat# MBS666960

CD252 Antibody – Mouse Monoclonal

Name: CD252 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666960
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666960

Human CD252 (CD134L, OX40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 binds to CD134 (OX40) present on activated T cells, providing a costimulatory signal (1). This… More details

$605 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD252, Monoclonal Antibody

Full Product Name

Monoclonal anti-human CD252 (CD134L, OX40L) /APC

Product Gene Name

anti-CD252 antibody

Product Synonym Names

anti-CD252 (OX40L); anti-human CD252 (OX40L); CD252; OX40 Ligand; gp34; anti-OX40L; anti-OX40 Ligand

Clonality

Monoclonal

Isotype

Mouse IgG1 kappa

Clone Number

ANC10G1

Host

Mouse

Species Reactivity

Human

Form/Format

50mM Sodium Phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).

Concentration

Volume in Vial: 0.2 ml (lot specific)

Specificity

Antibody ANC10G1 binds to CD252 on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

Preparation and Storage

Store at 2-5 degree C. Do not freeze! Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Immunogen

Human HUVEC and Recombinant soluble human CD252

Conjugation

Allophycocyanin (APC)

Production

Protein A purified antibody from tissue culture supernatant was conjugated to cross-linked Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusionchromatography. Antibody conjugate is at 1.0mg/ml with an A650/A280 ratio of 1.94.

Performance

Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, afterwhich 5 x 105 cells per tube were washed and incubated 45minutes on ice with 80ul of anti-CD252/APC at a 1:50dilution (20ug/ml). Cells were washed three times, fixedand analyzed by FACS. Cell stained positive with a meanshift of 1.2 log10 fluorescent units when compared to aMouse IgG1/APC negative control (#MBS666633). Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD252 antibody (#MBS666348).

Applications Tested/Suitable for anti-CD252 antibody

Flow Cytometry (FC/FACS) (9), ELISA (EIA)

Application Notes for anti-CD252 antibody

Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

Product References and Citations for anti-CD252 antibody

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763.
2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493.
3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.
4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.
5) V. Malmstrom, et al, (2001) J Immunol 166: 6972.
6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115.

Other Notes

Small volumes of anti-CD252 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD252 purchase

MBS666960 is an antibody targeting CD252. Also known as Monoclonal anti-human CD252 (CD134L, OX40L) /APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD252. It may not necessarily be applicable to this product.

Protein Family

Tumor necrosis factor ligand superfamily

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.