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B7-H4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668282

B7-H4, a member of B7 family, is a transmembrane protein that has been shown to inhibit T cell responses and neutrophil expansion during bacterial infections. B7-H4 mRNA is widely distributed in mouse and human peripheral tissues and can be induced in… More details

$210 / 0.025 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
B7-H4, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to B7-H4 (Clone: ABM53A6)
Product Gene Name
anti-B7-H4 antibody
Product Synonym Names
V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; Monoclonal antibody to B7H4 (Clone: ABM53A6)
Clonality
Monoclonal
Isotype
IgG2a k
Clone Number
ABM53A6
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4°C, stable for 6 months.
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.
OMIM
AY280972 mRNA
3D Structure
ModBase 3D Structure for Q7Z7D3
Immunogen Information
A partial length recombinant protein (a.a 23-220) of B7-H4 was used as the immunogen for this antibody.
Antigen
B7H4
Conjugate
Unconjugated

Product Categories/Family for anti-B7-H4 antibody

Antibodies; Immunology
Applications Tested/Suitable for anti-B7-H4 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-B7-H4 antibody
FACS: 0.5-1 ug/10^6 cells
IHC: 5 ug/ml
WB: 2-4 ug/ml

Immunohistochemistry (IHC) of anti-B7-H4 antibody

Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml
Mouse Anti-B7-H4 Monoclonal Antibody – Cat# MBS668282

Flow Cytometry (FC/FACS) of anti-B7-H4 antibody

Fig-2: Cell surface Flow analysis of B7-H4 antibody in human PBMC (Monocytes) cells using 0.5 ug/ 10^6 cells of anti-B7-H4 antibody (ABM53A6). Green represents isotype control;red represents anti-B7H4 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
Mouse Anti-B7-H4 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS668282

Western Blot (WB) of anti-B7-H4 antibody

Fig-3: Western blot analysis of B7H4. Anti-B7H4 antibody (Clone: ABM53A6) was used at 4 ug/ml on human B7H4-FC fusion protein Lysate.
Mouse Anti-B7-H4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS668282

Western Blot(WB) of anti-B7-H4 antibody

Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 ug/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates.
Mouse Anti-B7-H4 Monoclonal Antibody, Western Blot(WB) – Cat# MBS668282

Other Notes

Small volumes of anti-B7-H4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B7-H4 purchase

MBS668282 is an antibody targeting B7-H4. Also known as Monoclonal Antibody to B7-H4 (Clone: ABM53A6). Reactive in Mouse, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for B7-H4. It may not necessarily be applicable to this product.

NCBI GI #
74759262
NCBI GeneID
79679
NCBI Accession #
Q7Z7D3.1
UniProt Primary Accession #
Q7Z7D3
UniProt Secondary Accession #
Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
UniProt Related Accession #
Q7Z7D3
NCBI Official Full Name
V-set domain-containing T-cell activation inhibitor 1
NCBI Official Synonym Full Names
V-set domain containing T-cell activation inhibitor 1
NCBI Official Symbol
VTCN1
NCBI Official Synonym Symbols
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
UniProt Protein Name
V-set domain-containing T-cell activation inhibitor 1
UniProt Synonym Protein Names
B7 homolog 4; B7-H4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x
UniProt Gene Name
VTCN1
UniProt Synonym Gene Names
B7-H4
NCBI Protein Information
V-set domain-containing T-cell activation inhibitor 1

NCBI Summary for B7-H4

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for B7-H4

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1-p12
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Research Articles on B7-H4

1. study provided the first evidence that B7-H4 facilitated esophageal squamous cell carcinoma cell proliferation through promoting IL-6/STAT3 positive loopback pathway activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-B7-H4 antibody

 Reference Product  PubMed Publications
 FOXP3 antibody  >9 publications with B7-H4 and FOXP3
 CTLA4 antibody  >6 publications with B7-H4 and CTLA4
 BTLA antibody  >4 publications with B7-H4 and BTLA
 PDCD1LG2 antibody  >3 publications with B7-H4 and PDCD1LG2
 ICOSLG antibody  >2 publications with B7-H4 and ICOSLG
 TREML2 antibody  >1 publications with B7-H4 and TREML2

Pathways associated with anti-B7-H4 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-B7-H4 antibody

 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >20 publications with B7-H4 and Ovarian Neoplasms
 Disease Models, Animal Antibodies  >15 publications with B7-H4 and Disease Models, Animal
 Breast Neoplasms Antibodies  >14 publications with B7-H4 and Breast Neoplasms
 Inflammation Antibodies  >10 publications with B7-H4 and Inflammation
 Neoplasm Metastasis Antibodies  >10 publications with B7-H4 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >9 publications with B7-H4 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >9 publications with B7-H4 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >6 publications with B7-H4 and Kidney Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with B7-H4 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >6 publications with B7-H4 and Necrosis

Organs/Tissues associated with anti-B7-H4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >11 publications with B7-H4 and Lung
 Pancreas Antibodies  >10 publications with B7-H4 and Pancreas
 Kidney Antibodies  >7 publications with B7-H4 and Kidney
 Liver Antibodies  >6 publications with B7-H4 and Liver
 Bone Antibodies  >5 publications with B7-H4 and Bone
 Uterus Antibodies  >4 publications with B7-H4 and Uterus
 Prostate Antibodies  >3 publications with B7-H4 and Prostate
 Ovary Antibodies  >3 publications with B7-H4 and Ovary
 Placenta Antibodies  >1 publications with B7-H4 and Placenta
 Bladder Antibodies  >1 publications with B7-H4 and Bladder
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