• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD28 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668294

CD28 is a type I transmembrane protein that binds through its extracellular region to B7 proteins (CD80 and CD86), which are transmembrane proteins expressed on the surface of APCs (Antigen-Presenting Cells) and are up-regulated by inflammatory signals. CD28… More details

$190 / 0.025 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to CD28 (Clone: CB28)
Product Gene Name
anti-CD28 antibody
Product Synonym Names
T-cell-specific surface glycoprotein CD28; TP44; CD28
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
CB28
Host
Mouse
Species Reactivity
Human
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
26,186 Da
OMIM
J02988 mRNA
3D Structure
ModBase 3D Structure for P10747
Immunogen Information
A partial length recombinant protein from CD28 was used as the immunogen for this antibody.
Antigen
CD28

Product Categories/Family for anti-CD28 antibody

Antibodies; Immunology
Applications Tested/Suitable for anti-CD28 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD28 antibody
Flow Cytometry: 2-4 ug/10^6 cells

Flow Cytometry (FC/FACS) of anti-CD28 antibody

Fig-1: Cell surface Flow analysis of hCD2 in Jurkat cells using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody !0-4137). Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).
Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS668294

Flow Cytometry (FC/FACS) of anti-CD28 antibody

Fig-2: Cell surface Flow analysis of CD28 in PBMC (lymphocytes gated) using 2 ug/ 10^6 cells. Green represents isotype control ;red represents anti-hCD28 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).
Mouse Anti-CD28 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS668294

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD28 purchase

MBS668294 is an antibody targeting CD28. Also known as Monoclonal Antibody to CD28 (Clone: CB28). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
115973
NCBI GeneID
940
NCBI Accession #
P10747.1
UniProt Primary Accession #
P10747
UniProt Secondary Accession #
Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
UniProt Related Accession #
P10747
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
TP44; CD_antigen: CD28
Protein Family
T-cell-specific surface glycoprotein
UniProt Gene Name
CD28
NCBI Protein Information
T-cell-specific surface glycoprotein CD28

NCBI Summary for CD28

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

UniProt Comments for CD28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33.2

Cellular Component: cell surface; cytosol; external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: coreceptor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; SH3/SH2 adaptor activity

Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; phosphoinositide-mediated signaling; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of mitosis; positive regulation of T cell proliferation; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulatory T cell differentiation; T cell costimulation
Show more

Research Articles on CD28

1. A highly recurrent novel missense mutation in CD28 among angioimmunoblastic T-cell lymphoma patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD28 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD28 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1215 publications with CD28 and Autoimmune Diseases
 Necrosis Antibodies  >835 publications with CD28 and Necrosis
 Inflammation Antibodies  >777 publications with CD28 and Inflammation
 HIV Infections Antibodies  >449 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >354 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >307 publications with CD28 and Lung Diseases
 Lymphoma Antibodies  >277 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >224 publications with CD28 and Carcinoma
 Neoplasms, Experimental Antibodies  >204 publications with CD28 and Neoplasms, Experimental
 Liver Diseases Antibodies  >161 publications with CD28 and Liver Diseases

Organs/Tissues associated with anti-CD28 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7978 publications with CD28 and Blood
 Thymus Antibodies  >336 publications with CD28 and Thymus
 Brain Antibodies  >113 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >34 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >24 publications with CD28 and Connective Tissue
 Placenta Antibodies  >19 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >7 publications with CD28 and Adipose Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000