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Il-3ra (IL3RA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668414

Interleukin-3 receptor subunit alpha (IL-3RA) or CD123 is a high-affinity receptor for interleukin-3 (IL-3), which along with the primary signaling common Beta (Beta c) subunit (CD131) forms a heterodimer, enabling cell signaling. IL-3RA chain is a… More details

$210 / 0.025 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL-3RA (IL3RA), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to IL-3RA (Clone: ABM25C1)
Product Gene Name
anti-IL3RA antibody
Product Synonym Names
Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; CD123; IL-3R-alpha
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
ABM25C1
Host
Mouse
Species Reactivity
Human
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
34,466 Da
OMIM
M74782 mRNA
3D Structure
ModBase 3D Structure for P26951
Immunogen Information
A partial length recombinant protein (a.a 120-323) of IL-3RA was used as the immunogen for this antibody.
Antigen
IL-3RA

Product Categories/Family for anti-IL3RA antibody

Antibodies; Immunology
Applications Tested/Suitable for anti-IL3RA antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-IL3RA antibody
WB: 6-8 ug/ml
Flow Cytometry: 0.5-1 ug/10^6

Western Blot (WB) of anti-IL3RA antibody

Fig-1: Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 ug/ml on hbrain lysate.
Mouse Anti-IL3RA Monoclonal Antibody – Cat# MBS668414

Flow Cytometry (FC/FACS) of anti-IL3RA antibody

Fig-2: Cell surface flow analysis of human IL3-RA in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM25C1). Green represents isotype control; red represents anti-IL3 RA antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).
Mouse Anti-IL3RA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS668414

Other Notes

Small volumes of anti-IL3RA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL3RA purchase

MBS668414 is an antibody targeting IL3RA. Also known as Monoclonal Antibody to IL-3RA (Clone: ABM25C1). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL3RA. It may not necessarily be applicable to this product.

NCBI GI #
417184
NCBI GeneID
3563
NCBI Accession #
P26951.1
UniProt Primary Accession #
P26951
UniProt Secondary Accession #
Q5HYQ7; Q5HYQ8; Q9UEH7; A8K3F3; B9VI81
UniProt Related Accession #
P26951
NCBI Official Full Name
Interleukin-3 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Synonym Protein Names
CD_antigen: CD123
Protein Family
Interleukin-3 receptor
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
NCBI Protein Information
interleukin-3 receptor subunit alpha

NCBI Summary for IL3RA

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
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UniProt Comments for IL3RA

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2

Cellular Component: plasma membrane

Molecular Function: interleukin-3 receptor activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: MAPKKK cascade
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Research Articles on IL3RA

1. Data indicate that CD123 was preferentially expressed among CD33/CD117-positive precursor-T-acute lymphoblastic leukemia (T-ALL).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL3RA antibody

 Reference Product  PubMed Publications
 CSF2RB antibody  >9 publications with IL3RA and CSF2RB
 IL3 antibody  >8 publications with IL3RA and IL3
 JAK2 antibody  >2 publications with IL3RA and JAK2
 JAK1 antibody  >1 publications with IL3RA and JAK1

Pathways associated with anti-IL3RA antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Beta:gamma Signalling Through PI3Kgamma Pathway antibodies  G Beta:gamma Signalling Through PI3Kgamma Pathway Diagram
 G-protein Beta:gamma Signalling Pathway antibodies  G-protein Beta:gamma Signalling Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPVI-mediated Activation Cascade Pathway antibodies  GPVI-mediated Activation Cascade Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-IL3RA antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >9 publications with IL3RA and Necrosis
 Nervous System Diseases Antibodies  >5 publications with IL3RA and Nervous System Diseases
 Carcinoma Antibodies  >5 publications with IL3RA and Carcinoma
 Vascular Diseases Antibodies  >3 publications with IL3RA and Vascular Diseases
 Cardiovascular Diseases Antibodies  >3 publications with IL3RA and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with IL3RA and Inflammation
 Liver Diseases Antibodies  >2 publications with IL3RA and Liver Diseases
 Disease Models, Animal Antibodies  >2 publications with IL3RA and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >2 publications with IL3RA and Neoplasms, Experimental
 Lung Diseases Antibodies  >2 publications with IL3RA and Lung Diseases

Organs/Tissues associated with anti-IL3RA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >104 publications with IL3RA and Blood
 Brain Antibodies  >6 publications with IL3RA and Brain
 Embryonic Tissue Antibodies  >4 publications with IL3RA and Embryonic Tissue
 Spleen Antibodies  >3 publications with IL3RA and Spleen
 Liver Antibodies  >3 publications with IL3RA and Liver
 Intestine Antibodies  >2 publications with IL3RA and Intestine
 Placenta Antibodies  >1 publications with IL3RA and Placenta
 Pancreas Antibodies  >1 publications with IL3RA and Pancreas
 Testis Antibodies  >1 publications with IL3RA and Testis
 Prostate Antibodies  >1 publications with IL3RA and Prostate
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