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CARD8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668564

This antibody recognizes TUCAN, a 431 amino acid protein. TUCAN (tumor up-regulated CARD-containing antagonist of caspase nine) also known as CARD8 is a CARD domain containing protein. Proteins containing a CARD (caspase-associated recruitment domain) domain… More details

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Product Overview

Online Datasheet in PDF
Product Name
CARD8, Polyclonal Antibody
Full Product Name
Polyclonal Antibody to CARD8 (Tucan)
Product Gene Name
anti-CARD8 antibody
Product Synonym Names
CARD8; KIAA0955; NDPP1; Apoptotic protein NDPP1; CARDINAL; CARD-inhibitor of NF-kappa-B-activating ligand; DACAR; TUCAN; Tumor up-regulated CARD-containing antagonist of CASP9; Antibody to CARD8 (Tucan); Anti-CARD8 (Tucan) Antibody; CARD8 (Tucan) Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
50 ul sera
Purity/Purification
Serum
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
60,652 Da
OMIM
AB023172 mRNA
3D Structure
ModBase 3D Structure for Q9Y2G2
Immunogen Information
Full-length recombinant protein of human TUCAN (CARD8) was used as immunogen for this antibody
Antigen
CARD8 (Tucan)

Product Categories/Family for anti-CARD8 antibody

Antibodies; Apoptosis
Applications Tested/Suitable for anti-CARD8 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
Application Notes for anti-CARD8 antibody
WB: 1: 1000-1: 2000
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200

Western Blot (WB) of anti-CARD8 antibody

Fig: 1 Western blot analysis of TUCAN/CARD8 in tumor cell lines using at 1: 2000. Cell lysates were normalized for total protein content.
Rabbit Anti-CARD8 Polyclonal Antibody – Cat# MBS668564

Immunohistochemistry (IHC) of anti-CARD8 antibody

Fig: 2 Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1: 2000 using at 1: 2000 . A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-eosin counterstain.
Rabbit Anti-CARD8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS668564

Other Notes

Small volumes of anti-CARD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CARD8 purchase

MBS668564 is an antibody targeting CARD8. Also known as Polyclonal Antibody to CARD8 (Tucan). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CARD8. It may not necessarily be applicable to this product.

NCBI GI #
14424229
NCBI GeneID
22900
NCBI Accession #
Q9Y2G2.1
UniProt Primary Accession #
Q9Y2G2
UniProt Secondary Accession #
Q6PGP8; Q96P82; B5KVR6; B7Z496; B7Z4A2; E9PEM7; G3XAM9
UniProt Related Accession #
Q9Y2G2
NCBI Official Full Name
Caspase recruitment domain-containing protein 8
NCBI Official Synonym Full Names
caspase recruitment domain family member 8
NCBI Official Symbol
CARD8
NCBI Official Synonym Symbols
NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
UniProt Protein Name
Caspase recruitment domain-containing protein 8
UniProt Synonym Protein Names
Apoptotic protein NDPP1; CARD-inhibitor of NF-kappa-B-activating ligand; CARDINAL; DACAR; Tumor up-regulated CARD-containing antagonist of CASP9; TUCAN
Protein Family
Caspase recruitment domain-containing protein
UniProt Gene Name
CARD8
UniProt Synonym Gene Names
KIAA0955; NDPP1; CARDINAL; TUCAN
NCBI Protein Information
caspase recruitment domain-containing protein 8

NCBI Summary for CARD8

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
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UniProt Comments for CARD8

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: caspase activator activity; NACHT domain binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion
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Research Articles on CARD8

1. The NLRP3 rs10754558 gene polymorphism was significantly associated with the occurrence of CAD, while the CARD8 rs2043211 gene polymorphism was not involved.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CARD8 antibody

 Reference Product  PubMed Publications
 NLRP3 antibody  >34 publications with CARD8 and NLRP3
 CASP1 antibody  >22 publications with CARD8 and CASP1
 CASP9 antibody  >6 publications with CARD8 and CASP9
 PYCARD antibody  >4 publications with CARD8 and PYCARD
 CASP5 antibody  >2 publications with CARD8 and CASP5
 CARD6 antibody  >2 publications with CARD8 and CARD6
 NLRP2 antibody  >2 publications with CARD8 and NLRP2
 MEFV antibody  >1 publications with CARD8 and MEFV

Pathways associated with anti-CARD8 antibody

 Products by Pathway  Pathway Diagram
 NOD Pathway antibodies  NOD Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram

Diseases associated with anti-CARD8 antibody

 Disease Name  Pubmed Publications
 Gastrointestinal Diseases Antibodies  >14 publications with CARD8 and Gastrointestinal Diseases
 Necrosis Antibodies  >14 publications with CARD8 and Necrosis
 Immune System Diseases Antibodies  >14 publications with CARD8 and Immune System Diseases
 Musculoskeletal Diseases Antibodies  >11 publications with CARD8 and Musculoskeletal Diseases
 Nervous System Diseases Antibodies  >5 publications with CARD8 and Nervous System Diseases
 Respiratory Tract Diseases Antibodies  >4 publications with CARD8 and Respiratory Tract Diseases
 Fibrosis Antibodies  >2 publications with CARD8 and Fibrosis
 Heart Diseases Antibodies  >2 publications with CARD8 and Heart Diseases
 Ataxia Antibodies  >1 publications with CARD8 and Ataxia
 Breast Neoplasms Antibodies  >1 publications with CARD8 and Breast Neoplasms

Organs/Tissues associated with anti-CARD8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with CARD8 and Blood
 Lung Antibodies  >4 publications with CARD8 and Lung
 Brain Antibodies  >4 publications with CARD8 and Brain
 Muscle Antibodies  >3 publications with CARD8 and Muscle
 Kidney Antibodies  >3 publications with CARD8 and Kidney
 Vascular Antibodies  >3 publications with CARD8 and Vascular
 Prostate Antibodies  >2 publications with CARD8 and Prostate
 Liver Antibodies  >2 publications with CARD8 and Liver
 Intestine Antibodies  >2 publications with CARD8 and Intestine
 Spleen Antibodies  >2 publications with CARD8 and Spleen
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