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Spectrin Beta 3 (SPTBN2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS669029

Beta-III spectrin is a protein encoded by the gene SPTBN2, spans 2390 amino acids and consists of an amino-terminal actin binding domain (ABD), a central region containing seventeen spectrin repeat domains, and a carboxy-terminal pleckstrin homology domain.… More details

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Product Overview

Online Datasheet in PDF
Product Name
Spectrin beta 3 (SPTBN2), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16)
Product Gene Name
anti-SPTBN2 antibody
Product Synonym Names
Spectrin beta chain; non-erythrocytic 2; Beta-III spectrin; Spinocerebellar ataxia 5 protein
Clonality
Monoclonal
Isotype
IgG2a, Kappa
Clone Number
ABM5A16
Host
Mouse
Species Reactivity
Human
Form/Format
Purified
25 ug in 50 uL/100 ug in 200 uL in PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4°C; stable for 6 months.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
268,309 Da
OMIM
AB008567 mRNA
3D Structure
ModBase 3D Structure for O15020
Immunogen
A partial length recombinant protein (a.a 1280-1450) of Spectrin beta 3 was used as the immunogen for this antibody.

Product Categories/Family for anti-SPTBN2 antibody

Cancer Marker
Applications Tested/Suitable for anti-SPTBN2 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-SPTBN2 antibody
WB: 0.1-0.5 ug/ml
FACS: 2-4 ug/106

Western Blot (WB) of anti-SPTBN2 antibody

Figure-1: Western blot analysis of Spectrin beta 3. Anti-Spectrin beta 3 antibody (Clone: ABM5A16) was used at 0.1 ug/ml on h Kidney and h Brain lysates.
Mouse Anti-SPTBN2 Monoclonal Antibody – Cat# MBS669029

Flow Cytometry (FC/FACS) of anti-SPTBN2 antibody

Figure-2: Intracellular flow analysis of human Spectrin beta 3 in A431 cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
Mouse Anti-SPTBN2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS669029

Flow Cytometry (FC/FACS) of anti-SPTBN2 antibody

Figure-3: Intracellular flow analysis of human Spectrin beta 3 in HeLa cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
Mouse Anti-SPTBN2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS669029

Other Notes

Small volumes of anti-SPTBN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPTBN2 purchase

MBS669029 is an antibody targeting SPTBN2. Also known as Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTBN2. It may not necessarily be applicable to this product.

NCBI GI #
5902122
NCBI GeneID
6712
NCBI Accession #
NP_008877.1
UniProt Primary Accession #
O15020
UniProt Secondary Accession #
O14872; O14873
UniProt Related Accession #
O15020
GenBank Nucleotide #
NM_006946.3
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 2
NCBI Official Synonym Full Names
spectrin beta, non-erythrocytic 2
NCBI Official Symbol
SPTBN2
NCBI Official Synonym Symbols
SCA5; SCAR14; GTRAP41
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 2
UniProt Synonym Protein Names
Beta-III spectrin; Spinocerebellar ataxia 5 protein
Protein Family
Spectrin
UniProt Gene Name
SPTBN2
UniProt Synonym Gene Names
KIAA0302; SCA5
NCBI Protein Information
spectrin beta chain, non-erythrocytic 2

NCBI Summary for SPTBN2

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]
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UniProt Comments for SPTBN2

Probably plays an important role in neuronal membrane skeleton.

Research Articles on SPTBN2

1. Cardiac beta2-spectrin and downstream molecules are regulated in multiple forms of cardiovascular disease via Ca(2+)- and calpain-dependent proteolysis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SPTBN2 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
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 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
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 Interaction Between L1 And Ankyrins Pathway antibodies  Interaction Between L1 And Ankyrins Pathway Diagram
 L1CAM Interactions Pathway antibodies  L1CAM Interactions Pathway Diagram
 NCAM Signaling For Neurite Out-growth Pathway antibodies  NCAM Signaling For Neurite Out-growth Pathway Diagram
 NCAM Signaling For Neurite Out-growth Pathway antibodies  NCAM Signaling For Neurite Out-growth Pathway Diagram

Diseases associated with anti-SPTBN2 antibody

 Disease Name  Pubmed Publications
 Spinocerebellar Ataxias Antibodies  >16 publications with SPTBN2 and Spinocerebellar Ataxias
 Nervous System Diseases Antibodies  >15 publications with SPTBN2 and Nervous System Diseases
 Atrophy Antibodies  >5 publications with SPTBN2 and Atrophy
 Lung Diseases Antibodies  >1 publications with SPTBN2 and Lung Diseases

Organs/Tissues associated with anti-SPTBN2 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >8 publications with SPTBN2 and Nerve
 Brain Antibodies  >6 publications with SPTBN2 and Brain
 Eye Antibodies  >2 publications with SPTBN2 and Eye
 Blood Antibodies  >1 publications with SPTBN2 and Blood
 Prostate Antibodies  >1 publications with SPTBN2 and Prostate
 Liver Antibodies  >1 publications with SPTBN2 and Liver
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