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CK19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS669130

Keratin-19 (K19) is a type I intermediate filament protein and one of the best characterized of the keratins expressed in mature striated muscle. Keratin-19 is expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles.… More details

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Product Overview

Product Name
CK19, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to CK19 (Clone: A53-B/A2)
Product Gene Name
Product Synonym Gene Name
KRT19
Product Synonym Names
KRT19; Cytokeratin-19; K19; Keratin-19
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
A53-B/A2
Host
Mouse
Species Reactivity
Human
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Molecular Weight
44,106 Da
OMIM
J03607 Genomic DNA
3D Structure
ModBase 3D Structure for P08727
Antigen
CK19
Immunogen Information
Human breast cancer MCF-7 cells were used as the immunogen for the Keratin-19 antibody.
Conjugation
Unconjugated

Product Categories/Family for anti-CK19 antibody

Applications Tested/Suitable for anti-CK19 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CK19 antibody
WB analysis: 1-3 ug/ml
IHC analysis: 1: 100-1: 200

Western Blot (WB) of anti-CK19 antibody

Fig: 1 Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 ug/ml in 1) HepG2 and 2) MCF7 lysates.
Mouse Anti-CK19 Monoclonal Antibody – Cat# MBS669130

Immunohistochemistry (IHC) of anti-CK19 antibody

Fig: 2 Immunohistochemical analysis of CK19 in human colon cancer using CK19 antibody (Clone: A53-B/A2) at 1: 100 dilution.
Mouse Anti-CK19 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS669130

Immunohistochemistry (IHC) of anti-CK19 antibody

Fig: 3 Immunohistochemical analysis of CK19 in human Pancrease, Duct using CK19 antibody (Clone: A53-B/A2) at 10 ug/ml.
Mouse Anti-CK19 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS669130

Other Notes

Small volumes of anti-CK19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK19 purchase

MBS669130 is an antibody targeting CK19. Also known as Monoclonal Antibody to CK19 (Clone: A53-B/A2). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CK19. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 19
NCBI Official Synonym Full Names
keratin 19
NCBI Official Symbol
KRT19
NCBI Official Synonym Symbols
K19; CK19; K1CS
UniProt Protein Name
Keratin, type I cytoskeletal 19
UniProt Synonym Protein Names
Cytokeratin-19; CK-19; Keratin-19; K19
UniProt Gene Name
KRT19
UniProt Synonym Gene Names
CK-19; K19
NCBI Protein Information
keratin, type I cytoskeletal 19

NCBI Summary for CK19

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
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UniProt Comments for CK19

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CK19 antibody

Pathways associated with anti-CK19 antibody

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