MIF (Macrophage Migration Inhibitory Factor) (MIF) ELISA Kit for Human

This immunoassay kit allows for the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.
This assay employs an antibody specific for Anti-human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Small volumes of MIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS669149 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIF (Macrophage migration inhibitory factor) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIF. The ELISA analytical biochemical technique of the MBS669149 kit is based on MIF antibody-MIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).