MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha) (MIP-1alpha/CCL3) ELISA Kit for Human

This immunoassay kit allows for the in vitro quantitative determination of Huma MIP-1alpha/CCL3 concentrations in serum, plasma and other biological fluids.
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1 alpha/CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1alpha/CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1alpha/CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human MIP-1alpha/CCL3 can be calculated using the standard curve.

Small volumes of MIP-1alpha/CCL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS669176 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIP-1alpha/CCL3. The ELISA analytical biochemical technique of the MBS669176 kit is based on MIP-1alpha/CCL3 antibody-MIP-1alpha/CCL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIP-1alpha/CCL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIP-1alpha/CCL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).