Il-35 (Interleukin 35) (IL-35) ELISA Kit for Human

Background/Introduction: IL-35, acts as a potent anti-inflammatory cytokine, which has been shown to be required for the optimal suppressive activity of regulatory T-cell populations in both mice and man. This cytokine is a member of IL-12 family, and formed by pairing IL-12alpha (also known as p35) and Epstein-Barr virus-induced gene 3 (EBI3). IL-35 is produced primarily by CD4+Foxp3+Treg cells and required for the suppressive activity of regulatory T-cell populations. It is also produced by activated B cells, tolerogenic dendritic cells and to a lesser extent by activated endothelial cells, smooth muscle cells, and monocytes. Mechanistically, IL-35 signals in the target cells by binding to heterodimeric (gp130-IL-12Rbeta2) or homodimeric (gp130-gp130 or IL-12Rbeta2-IL-12Rbeta2) receptors to inhibit immunological responses. In contrast to other immunosuppressive cytokine, TGF-beta, IL-35 is not a constitutively expressed cytokine but rather a responsive cytokine induced by inflammatory stimuli. The loss of IL-35 expression reduces the in vivo suppressive capacity of Treg. However, little is known regarding the definitive mechanisms of IL-35 in autoimmune disease. Therefore we investigated the immunoregulatory role of IL-35 in patients with RA (Rheumatoid arthritis).

Small volumes of IL-35 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS669202 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-35 (Interleukin 35) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-35. The ELISA analytical biochemical technique of the MBS669202 kit is based on IL-35 antibody-IL-35 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-35 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-35. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).