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COVID 19 Spike S1 Coronavirus (COVID-19) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS669451

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of… More details

$670 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COVID 19 Spike S1 Coronavirus (COVID-19), Active Protein
Full Product Name
Recombinant SARS-CoV-2 Spike S1 Protein (His Tag)
Product Gene Name
Product Synonym Gene Name
2019-nCoV; SARS-CoV-2; SARS coronavirus 2 proteome; COVID19; SARS-CoV2
Product Synonym Names
2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1
Host
HEK293 cells
Form/Format
Lyophilized from a 0.22um filtered solution of PBS, pH7.4
Purity/Purification
>90% by SDS-PAGE.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Endotoxin
<1.0EU/ug of the protein by LAL method.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Biological Activity
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2ug/mL (100uL/well) can bind Recombinant nCoV-S1, the EC50 of nCoV-S1 is 0.25-0.45 ug/mL
Amino Acid note
The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1fused with a 6xHis tag at the C-terminus.

Product Categories/Family for COVID-19 active protein

Applications Tested/Suitable for COVID-19 active protein
Functional Assay

SDS-Page of COVID-19 active protein

Figure-1: Recombinant COVID-19 Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.
COVID-19 Active Protein – Cat# MBS669451

Testing Data of COVID-19 active protein

Fig 2 Immobilized Recombinant Human ACE2 at 2 ugmL (100uLwell) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 ugmL.
COVID-19 Active Protein, Testing Data – Cat# MBS669451

Other Notes

Small volumes of COVID-19 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COVID-19 purchase

MBS669451 is an active protein targeting COVID-19. Also known as Recombinant SARS-CoV-2 Spike S1 Protein (His Tag). Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for COVID-19. It may not necessarily be applicable to this product.

NCBI GeneID

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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