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DNMT2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS668323

DNMT2, also known as tRNA-aspartic acid methyltransferase 1 (TRDMT1), is highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNAAsp in many species, including lower eukaryotes, plants, insects, and humans. DNMT2 contains all… More details

$220 / 0.025 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNMT2, Monoclonal Antibody
Full Product Name
Monoclonal Antibody to DNMT2 (Clone: ABM1H70)
Product Gene Name
Product Synonym Names
HCG23994; isoform CRA_a; cDNA FLJ76302; Highly similar to Homo sapiens DNA (cytosine-5-)-methyltransferase 2 (DNMT2); tRNA aspartic acid methyltransferase 1 variant 3
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
ABM1H70
Host
Mouse
Species Reactivity
Mouse, Human
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Purity/Purification
Purified
Protein G Chromatography
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
3D Structure
ModBase 3D Structure for Q6ICS7
Immunogen Information
A partial length recombinant protein from DNMT2 was used as the immunogen for this antibody.
Antigen
DNMT2
Applications Tested/Suitable for anti-DNMT2 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-DNMT2 antibody
WB: 4-6 ug/ml
Flow Cytometry: 0.5-1 ug/10^6

Western Blot (WB) of anti-DNMT2 antibody

Fig-1: Western blot analysis of DNMT2. Anti-DNMTc2 antibody (Clone: ABM1H70) was used at 4 ug/ml on Jurkat and mouse embryonic liver lysates.
Mouse Anti-DNMT2 Monoclonal Antibody – Cat# MBS668323

Flow Cytometry (FC/FACS) of anti-DNMT2 antibody

Fig-2: Intracellular Flow analysis of DNMT2 antibody in Hela cells using 0.5 ug/ 10^6 cells of anti-DNMT2 antibody (ABM1H70). Green represents isotype control; red represents anti-DNMT 2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
Mouse Anti-DNMT2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS668323

Other Notes

Small volumes of anti-DNMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNMT2 purchase

MBS668323 is an antibody targeting DNMT2. Also known as Monoclonal Antibody to DNMT2 (Clone: ABM1H70). Reactive in Mouse, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DNMT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNMT2 protein
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
UniProt Protein Name
DNMT2 protein
UniProt Synonym Protein Names
DNMT2 protein
Protein Family
UniProt Gene Name
DNMT2
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase

NCBI Summary for DNMT2

This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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