Cytokeratin 8 (KRT8) Antibody – Mouse Monoclonal

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Ref.19

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Ref.13 Ref.14 Ref.17 Ref.19 Ref.20 Ref.22 Ref.30

Subcellular location: Cytoplasm. Nucleus › nucleoplasm

By similarity. Nucleus matrix

By similarity Ref.13 Ref.30.

Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Ref.13 Ref.19

Post-translational modification: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. Ref.12

Involvement in disease: Defects in KRT8 are a cause of cirrhosis (CIRRH) [

MIM:215600]. Ref.34

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.