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Cytokeratin 19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670019

MBS670019 is an antibody targeting Cytokeratin 19. Also known as Mouse Anti-Cytokeratin 19-HRP. Host: Mouse. More details

$475 / 1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 19, Monoclonal Antibody
Full Product Name
Mouse Anti-Cytokeratin 19-HRP
Product Synonym Names
Mouse Anti-Cytokeratin 19 - HRP; Mouse Anti-Cytokeratin 19
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2a
Clone Number
SB39g1
Host
Mouse
Form/Format
Horseradish Peroxidase (HRP) Conjugate
Specificity
Keratin 191
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology.2-6 Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7.7,8 Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin.
Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers.9,10
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2; store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.1 mg in 0.2 mL PBS/NaN3. Store at 2-8 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20.
Immunogen
Recombinant C-terminal keratin 19
Characterization
To ensure acceptable performance, each batch of product is tested ELISA, immunohistochemical staining (HT29 and HCC38 cells) or Western blotting (HT29 and HCC38 cell lysates) to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-Cytokeratin 19 antibody
ELISA; Immunohistochemistry; Western Blot
Application Notes for anti-Cytokeratin 19 antibody
Western Blot Suggested Dilution: 1:5,000-1:10,000
Immunohistochemistry Suggested Dilution: 1:1000-1:2000

Product References and Citations for anti-Cytokeratin 19 antibody

1) Lodish, H. et al. (2000) In: Molecular Cell Biology. NY, NY, W.H. Freeman and Co., pp 795-847.
3) Fuchs, E.V. et al. (1981) Cell 27: 75-84.
4) Hanukoglu, I. And E. Fuchs. (1983) Cell 33: 915-924.
5) Schiller, D.L. et al. (1982) EMBO J. 6:761-769.
6) Tseng, S.C.G. et al. (1982) Cell 30: 361-372.
7) Bosch, F.X. et al. (1988) J. Cell. Biol. 106:1635-1648.
8) Moll, R. et al. (1982) Cell 31: 11-24.
9) Frank, W.W. et al. (1983) Proc. Natl. Acad. Sci. USA 80:7113-7117.
10) Wu, F. et al. (2002) Internatl. J. Oncology 20: 31-37.

Other Notes

Small volumes of anti-Cytokeratin 19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cytokeratin 19. It may not necessarily be applicable to this product.

NCBI GI #
13537138
NCBI Official Full Name
cytokeratin 19
Protein Family
Keratin

UniProt Comments for Cytokeratin 19

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cytokeratin 19 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Cytokeratin 19 and FLNA

Pathways associated with anti-Cytokeratin 19 antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-Cytokeratin 19 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with Cytokeratin 19 and Bone
 Connective Tissue Antibodies  >4 publications with Cytokeratin 19 and Connective Tissue
 Heart Antibodies  >3 publications with Cytokeratin 19 and Heart
 Muscle Antibodies  >3 publications with Cytokeratin 19 and Muscle
 Brain Antibodies  >3 publications with Cytokeratin 19 and Brain
 Vascular Antibodies  >2 publications with Cytokeratin 19 and Vascular
 Embryonic Tissue Antibodies  >2 publications with Cytokeratin 19 and Embryonic Tissue
 Ear Antibodies  >1 publications with Cytokeratin 19 and Ear
 Kidney Antibodies  >1 publications with Cytokeratin 19 and Kidney
 Umbilical Cord Antibodies  >1 publications with Cytokeratin 19 and Umbilical Cord
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