Rat Anti-IL-10 Monoclonal Antibody – Cat# MBS670020

IL-10 Antibody – Rat Monoclonal

Name: IL-10 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS670020
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670020

MBS670020 is an antibody targeting IL-10. Also known as Rat Anti-Human IL-10-UNLB. Reactive in Human. Host: Rat. More details

$340 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL-10, Monoclonal Antibody

Full Product Name

Rat Anti-Human IL-10-UNLB

Product Gene Name

anti-IL-10 antibody

Product Synonym Names

Rat Anti-Human IL-10 - Purified, Unlabeled Antibody; Rat Anti-Human/Viral IL-10

Clonality

Monoclonal

Isotype

Rat IgG1 kappa

Clone Number

JES3-9D7

Host

Rat

Species Reactivity

Human

Form/Format

Purified (UNLB) Antibody

Specificity

Human and Viral Interleukin-10 (IL-10)1-5 (This is a neutralizing antibody)

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chromosome Location

NC_004002.1 (2243..2749)

Immunogen

COS-expressed human IL-10

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-IL-10 antibody

ELISA; Flow Cytometry; Immunofluorescence; Western Blot

Application Notes for anti-IL-10 antibody

Research Application(s): ELISA Capture: Purified JES3-9D7 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified JES3-9D7 antibody should be paired with biotinylated JES3-12G8 antibody as the detection antibody, with purified recombinant human IL-10 as the standard.

Immunofluorescence/Flow Cytometry: The JES3-9D7 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control.

Western Blotting: The purified JES3-9D7 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions.

Neutralization: The LE/AF form of JES3-9D7 can be used for neutralizing human/viral IL-10 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.

Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-IL-10 antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
2. Gotlieb, W.H., J.S. Abrams, J.M. Watson, T. Velu, J.S. Berek, and O. Martinez-Maza. 1992. Cytokine 4:385.
3. Yssel, H., R. de Waal Malefyt, M-G. Roncarolo, J. Abrams, R. Lahesmaa, H. Spits, and J. de Vries. 1992. J. Immunol. 149:2378.
4. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
5. Burdin, N., C. Peronne, J. Banchereau, and F. Rousset. 1993. J. Exp. Med. 177:295.

Other Notes

Small volumes of anti-IL-10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.

NCBI GI #

NCBI GeneID

NCBI Accession #

GenBank Nucleotide #

NCBI Official Full Name

IL-10

NCBI Official Symbol

SPPV_03

NCBI Protein Information

IL-10

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-IL-10 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with IL-10 and Bone
Connective Tissue Antibodies	>4 publications with IL-10 and Connective Tissue
Heart Antibodies	>3 publications with IL-10 and Heart
Muscle Antibodies	>3 publications with IL-10 and Muscle
Brain Antibodies	>3 publications with IL-10 and Brain
Vascular Antibodies	>2 publications with IL-10 and Vascular
Embryonic Tissue Antibodies	>2 publications with IL-10 and Embryonic Tissue
Ear Antibodies	>1 publications with IL-10 and Ear
Kidney Antibodies	>1 publications with IL-10 and Kidney
Umbilical Cord Antibodies	>1 publications with IL-10 and Umbilical Cord