Rat Anti-CSF2 Monoclonal Antibody – Cat# MBS670026

Gm-CSF (CSF2) Antibody – Rat Monoclonal

Name: CSF2 Antibody; Monoclonal; Rat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS670026
Price: 395 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670026

MBS670026 is an antibody targeting CSF2. Also known as Rat Anti-Human GM-CSF-UNLB. Reactive in Human. Host: Rat. More details

$395 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GM-CSF (CSF2), Monoclonal Antibody

Full Product Name

Rat Anti-Human GM-CSF-UNLB

Product Gene Name

anti-CSF2 antibody

Product Synonym Names

Rat Anti-Human GM-CSF - Purified, Unlabeled Antibody; Rat Anti-Human GM-CSF

Clonality

Monoclonal

Isotype

Rat IgG2a

Clone Number

BVD2-21C11

Host

Rat

Species Reactivity

Human

Form/Format

Purified (UNLB) Antibody

Specificity

Human granulocyte/macrophage - colony stimulating factor (GM-CSF)1-4 The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a neutralizing antibody.)

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.

Molecular Weight

16,295 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (131409485..131411859). Location: 5q31.1

OMIM

138960

3D Structure

ModBase 3D Structure for P04141

Immunogen

E. coli-expressed human GM-CSF

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-CSF2 antibody

ELISA; Flow Cytometry; Immunofluorescence

Application Notes for anti-CSF2 antibody

Research Application(s): ELISA Detection: Biotinylated BVD2-21C11 monoclonal antibody is the preferred detection antibody in a sandwich ELISA for quantifying human GM-CSF protein levels.1-4 Biotinylated BVD2-21C11antibody should be paired with purified BVD2-23B6 antibody as the capture antibody, with purified recombinant human GM-CSF as the standard.

Immunofluorescence/Flow Cytometry: BVD2-2C11 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate GM-CSF-positive cells within mixed cell populations. Recombinant human GM-CSF, unlabeled BVD2-21C11, or Rat IgG2a should be used as a specificity control.

Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-CSF2 antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133-157.
3. Bacchetta, R., T. de Wall Malefyt, H. Yssel, J.S. Abrams, J.E. de Vries, H. Spits, and M.G. Roncarolo. 1990, Host reactive CD4+ and CD8+ T cells clones isolated from a human chimera produce IL-5, Il-2, IFN-gamma and granulocyte/macrophage stimulating factor bit not IL-4. J. Immunol. 144:902-908.
4. Kita, H., T. Ohnishi, Y. Okubo, D. Weller, J.S. Abrams, and G.J. Gleich. 1991. GM-CSF and interleukin-3 release by human peripheral blood eosinophils and neutrophils. J. Exp. Med. 174:745-748.

Other Notes

Small volumes of anti-CSF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF2. It may not necessarily be applicable to this product.

NCBI GI #

183364

NCBI GeneID

1437

UniProt Primary Accession #

P04141

UniProt Secondary Accession #

Q14CE8; Q2VPI8; Q8NFI6

UniProt Related Accession #

P04141; Q647J8

NCBI Official Full Name

GM-CSF

NCBI Official Synonym Full Names

colony stimulating factor 2 (granulocyte-macrophage)

NCBI Official Symbol

CSF2

NCBI Official Synonym Symbols

GMCSF; MGC131935; MGC138897

UniProt Protein Name

Granulocyte-macrophage colony-stimulating factor

UniProt Synonym Protein Names

Colony-stimulating factor; CSF; Molgramostin; Sargramostim

Protein Family

Granulocyte-macrophage colony-stimulating factor

UniProt Gene Name

CSF2

UniProt Synonym Gene Names

GMCSF

UniProt Entry Name

CSF2_HUMAN

NCBI Protein Information

granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor

NCBI Summary for CSF2

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]

UniProt Comments for CSF2

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.

Research Articles on CSF2

1. The levels of EPO, SCF and GM-CSF in chronic aplastic anemia patients were obviously lower than those in the healthy individuals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CSF2 antibody

Reference Product	PubMed Publications
CSF2RB antibody	>6 publications with CSF2 and CSF2RB
CSF2RA antibody	>4 publications with CSF2 and CSF2RA
KITLG antibody	>2 publications with CSF2 and KITLG
IL3RA antibody	>2 publications with CSF2 and IL3RA
SDC2 antibody	>1 publications with CSF2 and SDC2

Pathways associated with anti-CSF2 antibody

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-CSF2 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>17 publications with CSF2 and Necrosis
Inflammation Antibodies	>14 publications with CSF2 and Inflammation
Neoplasms Antibodies	>10 publications with CSF2 and Neoplasms
Immune System Diseases Antibodies	>9 publications with CSF2 and Immune System Diseases
Respiratory Tract Diseases Antibodies	>6 publications with CSF2 and Respiratory Tract Diseases
Bacterial Infections Antibodies	>6 publications with CSF2 and Bacterial Infections
Leukemia Antibodies	>6 publications with CSF2 and Leukemia
Hematologic Diseases Antibodies	>6 publications with CSF2 and Hematologic Diseases
Lung Diseases Antibodies	>5 publications with CSF2 and Lung Diseases
Bone Marrow Diseases Antibodies	>5 publications with CSF2 and Bone Marrow Diseases

Organs/Tissues associated with anti-CSF2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>22 publications with CSF2 and Blood
Bone Antibodies	>6 publications with CSF2 and Bone
Lung Antibodies	>3 publications with CSF2 and Lung