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Il-7 (IL7) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670051

MBS670051 is an antibody targeting IL7. Also known as Rat Anti-Human IL-7-BIOT. Reactive in Human. Host: Rat. More details

$440 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-7 (IL7), Monoclonal Antibody
Full Product Name
Rat Anti-Human IL-7-BIOT
Product Gene Name
anti-IL7 antibody
Product Synonym Names
Rat Anti-Human IL-7 - Biotin; Rat Anti-Human IL-7
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
BVD10-11C10
Host
Rat
Species Reactivity
Human
Form/Format
Biotin (BIOT) Conjugate
Specificity
Human Interleukin-7 (IL-7)1,2 (This is a neutralizing antibody.)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Molecular Weight
20,187 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (79645005..79717758, complement). Location: 8q12-q13
OMIM
146660
3D Structure
ModBase 3D Structure for P13232
Immunogen
E. coli-expressed recombinant human IL-7
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-IL7 antibody
ELISA; Immunoprecipitation; Western Blot
Application Notes for anti-IL7 antibody
Research Application(s): ELISA Detection: BVD10-11C10 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying human IL-7 protein levels.1,2 Biotinylated BVD10-11C10 antibody should be paired with purified BVD10-40F6 antibody as the capture antibody, with purified recombinant human IL-7 as the standard.
Western Blotting: The purified BVD10-11C10 antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-7 under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-IL7 antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

Other Notes

Small volumes of anti-IL7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL7. It may not necessarily be applicable to this product.

NCBI GI #
4504677
NCBI GeneID
3574
NCBI Accession #
NP_000871.1
UniProt Primary Accession #
P13232
UniProt Secondary Accession #
A0N0L3
UniProt Related Accession #
P13232; Q5FBX5; Q5FBY3; Q5FBY5; Q5FBY6; Q5FBY8; Q5FBY9
GenBank Nucleotide #
NM_000880.3
NCBI Official Full Name
interleukin-7 isoform 1
NCBI Official Synonym Full Names
interleukin 7
NCBI Official Symbol
IL7
NCBI Official Synonym Symbols
IL-7
UniProt Protein Name
Interleukin-7
Protein Family
Interleukin
UniProt Gene Name
IL7
UniProt Entry Name
IL7_HUMAN
NCBI Protein Information
interleukin-7; OTTHUMP00000227044; OTTHUMP00000227046

NCBI Summary for IL7

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.
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UniProt Comments for IL7

Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-7/IL-9 family.

Research Articles on IL7

1. immunological nonresponders to HIV therapy display elevated BM IL-7/IL-7Ralpha expression but impaired T-cell responsiveness to IL-7, suggesting the activity of a central compensatory pathway targeted to replenish the CD4+ compartment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL7 antibody

 Reference Product  PubMed Publications
 IL7R antibody  >7 publications with IL7 and IL7R
 IL2RG antibody  >7 publications with IL7 and IL2RG

Pathways associated with anti-IL7 antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram

Diseases associated with anti-IL7 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >42 publications with IL7 and Neoplasms
 Lymphoma Antibodies  >18 publications with IL7 and Lymphoma
 Neoplasms, Experimental Antibodies  >14 publications with IL7 and Neoplasms, Experimental
 Lymphoma, T-Cell Antibodies  >13 publications with IL7 and Lymphoma, T-Cell
 Lymphopenia Antibodies  >13 publications with IL7 and Lymphopenia
 Inflammation Antibodies  >12 publications with IL7 and Inflammation
 Multiple Sclerosis Antibodies  >12 publications with IL7 and Multiple Sclerosis
 Demyelinating Diseases Antibodies  >11 publications with IL7 and Demyelinating Diseases
 Leukemia, Myeloid, Acute Antibodies  >5 publications with IL7 and Leukemia, Myeloid, Acute
 Carcinoma Antibodies  >5 publications with IL7 and Carcinoma

Organs/Tissues associated with anti-IL7 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >136 publications with IL7 and Blood
 Bone Antibodies  >36 publications with IL7 and Bone
 Thymus Antibodies  >33 publications with IL7 and Thymus
 Lymph Node Antibodies  >15 publications with IL7 and Lymph Node
 Skin Antibodies  >11 publications with IL7 and Skin
 Spleen Antibodies  >10 publications with IL7 and Spleen
 Liver Antibodies  >9 publications with IL7 and Liver
 Intestine Antibodies  >5 publications with IL7 and Intestine
 Brain Antibodies  >4 publications with IL7 and Brain
 Kidney Antibodies  >2 publications with IL7 and Kidney
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