• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Il-4 (IL4) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670066

MBS670066 is an antibody targeting IL4. Also known as Rat Anti-Mouse IL-4-UNLB. Reactive in Mouse. Host: Rat. More details

$340 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-4 (IL4), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse IL-4-UNLB
Product Gene Name
anti-IL4 antibody
Product Synonym Names
Rat Anti-Mouse IL-4 - Purified, Unlabeled Antibody; Rat Anti-Mouse IL-4
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
BVD4-1D11
Host
Rat
Species Reactivity
Mouse
Form/Format
Purified (UNLB) Antibody
Specificity
Mouse Interleukin-4 (IL-4)1, 2 (This is a neutralizing antibody.)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Molecular Weight
15,023 Da
Chromosome Location
Chromosome: 2; NC_010444.2 (140218906..140226454). Location: 2; 2
3D Structure
ModBase 3D Structure for Q04745
Immunogen
E. coli-expressed recombinant mouse IL-4
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-IL4 antibody
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
Application Notes for anti-IL4 antibody
Research Application(s): ELISA Capture: BVD4-1D11 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified BVD4-1D11 antibody should be paired with biotinylated BVD6-24G2 antibody as the detection antibody, with purified recombinant mouse IL-4 as the standard.
Immunofluorescence/Flow Cytometry: BVD4-1D11 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control.
Western Blotting: The purified BVD4-1D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
Neutralization: The LE/AF form of BVD4-1D11 can be used for neutralizing mouse IL-4 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-IL4 antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
3. Finkelman, F., K. Madden, S. Morris, J. Holmes, N. Bolani,, I. Jkatona, and C. Maliszewski. 1993. Anti-cytokine antibodies as carrier proteins. J. Immunol. 151:1235-1244.
4. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47-58.

Other Notes

Small volumes of anti-IL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL4. It may not necessarily be applicable to this product.

NCBI GI #
55742622
NCBI GeneID
397225
NCBI Accession #
NP_999288.1
UniProt Primary Accession #
Q04745
UniProt Secondary Accession #
Q29054; Q8MKE6
UniProt Related Accession #
Q04745
GenBank Nucleotide #
NM_214123.1
NCBI Official Full Name
interleukin-4
NCBI Official Symbol
IL4
UniProt Protein Name
Interleukin-4
UniProt Synonym Protein Names
B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
Protein Family
Interleukin
UniProt Gene Name
IL4
UniProt Entry Name
IL4_PIG
NCBI Protein Information
interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1

UniProt Comments for IL4

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-4/IL-13 family.
Show more

Research Articles on IL4

1. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL4 antibody

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-IL4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >237 publications with IL4 and Neoplasms
 Hypersensitivity Antibodies  >211 publications with IL4 and Hypersensitivity
 Inflammation Antibodies  >191 publications with IL4 and Inflammation
 Hypersensitivity, Immediate Antibodies  >167 publications with IL4 and Hypersensitivity, Immediate
 Asthma Antibodies  >144 publications with IL4 and Asthma
 Lung Diseases Antibodies  >134 publications with IL4 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >124 publications with IL4 and Respiratory Hypersensitivity
 Leukemia Antibodies  >64 publications with IL4 and Leukemia
 Lymphoma Antibodies  >56 publications with IL4 and Lymphoma
 Intestinal Diseases Antibodies  >53 publications with IL4 and Intestinal Diseases
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000