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Il-6 (IL6) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670075

MBS670075 is an antibody targeting Il6. Also known as Rat Anti-Mouse IL-6-LE/AF. Reactive in Mouse. Host: Rat. More details

$405 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-6 (Il6), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse IL-6-LE/AF
Product Gene Name
anti-Il6 antibody
Product Synonym Names
Rat Anti-Mouse IL-6 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-6
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
MP5-20F3
Host
Rat
Species Reactivity
Mouse
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Specificity
Mouse Interleukin-6 (IL-6)1-3 (This is a neutralizing antibody.)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Molecular Weight
24,357 Da
Chromosome Location
Chromosome: 4; NC_005103.2 (456799..461376, complement). Location: 4q11
3D Structure
ModBase 3D Structure for P20607
Immunogen
COS-7-expressed recombinant mouse IL-6
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-Il6 antibody
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
Application Notes for anti-Il6 antibody
Research Application(s): ELISA Capture: MP5-20F3 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-6 protein levels.3-5 Purified MP5-20F3 antibody should be paired with biotinylated MP5-32C11 antibody as the detection antibody, with purified recombinant mouse IL-6 as the standard.
Immunofluorescence/Flow Cytometry: MP5-20F3 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-6-positive cells within mixed cell populations.6 Recombinant mouse IL-6 or Rat IgG1 should be used as a specificity control.
Western Blotting: The purified MP5-20F3 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
Neutralization: The LE/AF form of MP5-20F3 can be used for neutralizing mouse IL-6 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-Il6 antibody

1. Suda, T., A. O'Garra, I. MacNeil, M. Fischer, M. Bond, and A. Zlotnik. 1990. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell. Immunol. 129:228-240.
2. Starnes, Jr., A., M. Pearce, A. Tewari, J. Kim, J,-C. Zou, and J. Abrams. 1990. Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-a challenge in mice. J. Immunol. 145:4185-4191.
3. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
6. Prussin, C., and D.D. Metcalf. 1995. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Meth. 188:117-128.

Other Notes

Small volumes of anti-Il6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Il6. It may not necessarily be applicable to this product.

NCBI GI #
7549769
NCBI GeneID
24498
NCBI Accession #
NP_036721.1
UniProt Primary Accession #
P20607
UniProt Related Accession #
P20607
GenBank Nucleotide #
NM_012589.1
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
UniProt Protein Name
Interleukin-6
Protein Family
Interleukin
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6
UniProt Entry Name
IL6_RAT
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)

NCBI Summary for Il6

a cytokine involved in development and possibly in neurodegenerative processes [RGD]

UniProt Comments for Il6

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.
Show more

Research Articles on Il6

1. Data identify IL-6 as an important target for modulation of autoimmune responses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Il6 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-Il6 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1069 publications with Il6 and Inflammation
 Neoplasms Antibodies  >539 publications with Il6 and Neoplasms
 Cardiovascular Diseases Antibodies  >454 publications with Il6 and Cardiovascular Diseases
 Lung Diseases Antibodies  >177 publications with Il6 and Lung Diseases
 Leukemia Antibodies  >133 publications with Il6 and Leukemia
 Atherosclerosis Antibodies  >126 publications with Il6 and Atherosclerosis
 Liver Diseases Antibodies  >123 publications with Il6 and Liver Diseases
 Obesity Antibodies  >122 publications with Il6 and Obesity
 Insulin Resistance Antibodies  >101 publications with Il6 and Insulin Resistance
 Kidney Diseases Antibodies  >98 publications with Il6 and Kidney Diseases

Organs/Tissues associated with anti-Il6 antibody

 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with Il6 and Uncharacterized Tissue
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