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Gm-CSF (CSF2) Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670099

MBS670099 is an antibody targeting CSF2. Also known as Rat Anti-Mouse GM-CSF-UNLB. Reactive in Mouse. Host: Rat. More details

$340 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GM-CSF (CSF2), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse GM-CSF-UNLB
Product Gene Name
anti-CSF2 antibody
Product Synonym Names
Rat Anti-Mouse GM-CSF - Purified, Unlabeled Antibody; Rat Anti-Mouse GM-CSF
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
MP1-31G6
Host
Rat
Species Reactivity
Mouse
Form/Format
Purified (UNLB) Antibody
Specificity
Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF)1-3 (This is a neutralizing antibody.)
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Molecular Weight
16,295 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (131409485..131411859). Location: 5q31.1
OMIM
138960
3D Structure
ModBase 3D Structure for P04141
Immunogen
Yeast-expressed recombinant mouse GM-CSF
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CSF2 antibody
ELISA; Immunoprecipitation; Western Blot
Application Notes for anti-CSF2 antibody
Research Application(s): ELISA Detection: MP1-31G6 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse GM-CSF protein levels.1,3-5 Biotinylated MP1-31G6 antibody should be paired with purified MP1-22E9 antibody as the capture antibody, with purified recombinant mouse GM-CSF as the standard.
Western Blotting: The purified MP1-22E9 antibody is also useful for immunoprecipitation4 and Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse GM-CSF under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-CSF2 antibody

1. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
2. Suda, T., A. O'Garra, I. MacNeil, M. Fischer, M. Bond, and A. Zlotnik. 1990. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell. Immunol. 129:228-240.
3. Nozaki, S., J. Abrams, M. Pearce, and D. Sauder. 1991. Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin-1 in Pam 212 keratinocytes. J. Invest. Dermatol. 97:10-14.
4. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

Other Notes

Small volumes of anti-CSF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF2. It may not necessarily be applicable to this product.

NCBI GI #
183364
NCBI GeneID
1437
UniProt Primary Accession #
P04141
UniProt Secondary Accession #
Q14CE8; Q2VPI8; Q8NFI6
UniProt Related Accession #
P04141; Q647J8
NCBI Official Full Name
GM-CSF
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
CSF2
NCBI Official Synonym Symbols
GMCSF; MGC131935; MGC138897
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF; Molgramostin; Sargramostim
Protein Family
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF
UniProt Entry Name
CSF2_HUMAN
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor

NCBI Summary for CSF2

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]
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UniProt Comments for CSF2

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.
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Research Articles on CSF2

1. The levels of EPO, SCF and GM-CSF in chronic aplastic anemia patients were obviously lower than those in the healthy individuals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CSF2 antibody

 Reference Product  PubMed Publications
 CSF2RB antibody  >6 publications with CSF2 and CSF2RB
 CSF2RA antibody  >4 publications with CSF2 and CSF2RA
 KITLG antibody  >2 publications with CSF2 and KITLG
 IL3RA antibody  >2 publications with CSF2 and IL3RA
 SDC2 antibody  >1 publications with CSF2 and SDC2

Pathways associated with anti-CSF2 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-CSF2 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >17 publications with CSF2 and Necrosis
 Inflammation Antibodies  >14 publications with CSF2 and Inflammation
 Neoplasms Antibodies  >10 publications with CSF2 and Neoplasms
 Immune System Diseases Antibodies  >9 publications with CSF2 and Immune System Diseases
 Respiratory Tract Diseases Antibodies  >6 publications with CSF2 and Respiratory Tract Diseases
 Bacterial Infections Antibodies  >6 publications with CSF2 and Bacterial Infections
 Leukemia Antibodies  >6 publications with CSF2 and Leukemia
 Hematologic Diseases Antibodies  >6 publications with CSF2 and Hematologic Diseases
 Lung Diseases Antibodies  >5 publications with CSF2 and Lung Diseases
 Bone Marrow Diseases Antibodies  >5 publications with CSF2 and Bone Marrow Diseases

Organs/Tissues associated with anti-CSF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with CSF2 and Blood
 Bone Antibodies  >6 publications with CSF2 and Bone
 Lung Antibodies  >3 publications with CSF2 and Lung
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