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fadd Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670127

MBS670127 is an antibody targeting fadd. Also known as Mouse Anti-Human FADD-UNLB. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
FADD, Monoclonal Antibody
Full Product Name
Mouse Anti-Human FADD-UNLB
Product Gene Name
Product Synonym Names
Mouse Anti-Human FADD - Purified, Unlabeled Antibody; Mouse Anti-Human FADD
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
1A11
Host
Mouse
Species Reactivity
Human
Form/Format
Purified (UNLB) Antibody
Specificity
Human FADD
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1 beta -converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.1-6
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The Sepharose 4B (SEPH) conjugate is supplied as 50 tests in 2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. Shake well before use.
Reagents are stable for the period shown on the label if stored as directed.
Chromosome Location
chromosome: Un;Â

Characterization
To insure lot-to-lot consistency, each batch of product is tested by western blotting and/or ELISA to conform with the characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-fadd antibody
ELISA; Immunoprecipitation; Western Blot
Application Notes for anti-fadd antibody
ELISA Suggested Dilution: 1:3,000-1:5,000

Product References and Citations for anti-fadd antibody

1. Chinnaiyan, A.M., K. O'Rourke, M. Tewari, and V.M. Dixit. 1995. Cel. 81:505.
2. Cleveland, J.L., and J.N. Ihle. 1995. Cell 81:479.
3. Muzio, M., A.M. Chinnaiyan, F.C. Kishkel, K. O'Rourke, A. Shevchenko, J. Ni, C. Scaffidi, J.D. Bretz, M. Zhang, R. Gentz, M. Mann, P.H. Krammer, M.E. Peter, and V.M. Dixit. 1996. Cell 85:817.
4. Wieder, T., F. Essmann, A. Prokop, K. Schmelz, K. Schulze-Osthoff, R. Beyaert, B. Dorken, and P.T. Daniel. 2001. Blood 97:1378.
5. Newton, K., A.W. Harris, M.L. Bath, K.G.C. Smith, and A. Strasser. 1998. EMBO J. 17:706.
6. Boldin, M.P., E.E. Varfolomeev, Z. Pancer, I.L. Meet, J.H. Camonis, and D. Wallach. 1995. J. Biol. Chem. 270:7795.

Other Notes

Small volumes of anti-fadd antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for fadd. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Official Full Name
FADD
NCBI Official Symbol
fadd
NCBI Protein Information
protein FADD

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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