Rat Anti-TNF Monoclonal Antibody – Cat# MBS670184

TNF Antibody – Rat Monoclonal

Name: TNF Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS670184
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670184

MBS670184 is an antibody targeting TNF-a. Also known as Rat Anti-Mouse TNF-a-FITC. Reactive in Mouse. Host: Rat. More details

$305 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TNF-a, Monoclonal Antibody

Full Product Name

Rat Anti-Mouse TNF-a-FITC

Product Synonym Names

Rat Anti-Mouse TNF-alpha

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

MP6-XT22

Host

Rat

Species Reactivity

Mouse

Form/Format

Fluorescein (FITC) Conjugate

Specificity

Mouse Tumor Necrosis Factor-alpha (TNF-alpha)

Preparation and Storage

The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.

Immunogen

E. coli-expressed recombinant mouse TNF-alpha

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Warning

Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Applications Tested/Suitable for anti-TNF-a antibody

ELISA, Western Blot

Application Notes for anti-TNF-a antibody

ELISA Capture: MP6-XT22 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse TNF-alpha protein levels. Purified MP6-XT22 antibody should be paired with a suitably labeled detection antibody as the detection antibody, with purified recombinant mouse TNF-alpha as the standard.; Flow Cytometry: FITC conjugated MP6-XT22 antibodies are useful for intracellular staining in cells producing TNF-alpha. Intracellular staining buffers are recommended for this application.; Western Blot: The purified MP6-XT22 antibody is also useful for Western Blot applications. For Western Blot, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <=100ng/lane of recombinant mouse TNF-alpha under reducing conditions.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-TNF-a antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Litton, M., B. Sander, E. Murphy, A. O'Garra, and J. Abrams. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J. Immunol. Meth. 175:47-58.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

Other Notes

Small volumes of anti-TNF-a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNF-a. It may not necessarily be applicable to this product.

Protein Family

Tumor necrosis factor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.