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CD5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670206

MBS670206 is an antibody targeting CD5. Also known as Mouse Anti-Human CD5-FITC. Reactive in Human. Host: Mouse. More details

$295 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD5, Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD5-FITC
Product Synonym Names
Mouse Anti-Human CD5
Clonality
Monoclonal
Isotype
IgG1
Clone Number
UCHT2
Host
Mouse
Species Reactivity
Human
Form/Format
Fluorescein (FITC) Conjugate
Specificity
Human CD5
CD5, a member of the scavenger receptor superfamily of cell surface receptors, is a type I transmembrane glycoprotein whose ligand is CD72. It is expressed on most thymocytes, all mature T cells, and a subset of B cells (B-1 cells). CD5 plays a role in regulation of T cell activation and signaling.1-5
Preparation and Storage
The fluorescein (FITC) conjugate is supplied as 100 tests in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-CD5 antibody
Flow Cytometry, Immunohistochemistry (Frozen sections), Western lotting, Immunoprecipitation
Application Notes for anti-CD5 antibody
Flow Cytometry: 20 uL/10^6 cells

Product References and Citations for anti-CD5 antibody

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD5 Section,Academic Press, New York, p. 143.
4. Lydyard, P.M., A. Lamour, L.E. MacKenzie, C. Jamin, R.A. Mageed, and P. Youinou. 1993. Immunol. Lett. 38:159.
5. Lankester, A.C., G.M. van Schijndel, J.L. Cordell, C.J. van Noesel, and R.A. van Lier. 1994. Eur. J. Immunol. 24:812.

Other Notes

Small volumes of anti-CD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
CD5 molecule, partial

UniProt Comments for CD5

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: cell proliferation; receptor-mediated endocytosis; T cell costimulation; cell recognition
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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