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CD44 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670247

MBS670247 is an antibody targeting CD44-SPRD. Also known as Mouse Anti-Human CD44-SPRD. Reactive in Human. Host: Mouse. More details

$405 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD44-SPRD, Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD44-SPRD
Product Synonym Names
Mouse Anti-Human CD44 (Pgp-1)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
F10-44-2
Host
Mouse
Species Reactivity
Human
Form/Format
Spectral Red (SPRD) Conjugate
Specificity
Human CD44
CD44, also known as phagocytic glycoprotein-1 (Pgp-1) and the receptor for hyaluronate, exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation.1-6
Preparation and Storage
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-CD44-SPRD antibody
Flow Cytometry, Immunohistochemistry (Frozen sections and Paraffin sections), Immunoprecipitation
Application Notes for anti-CD44-SPRD antibody
Flow Cytometry: 10 uL/10^6 cells

Product References and Citations for anti-CD44-SPRD antibody

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD44 Section, Academic Press, New York, p. 240.
5. Goldstein, L.A., and E.C. Butcher. 1990. Immunogenetics 32:389.
6. Lesley, J., R. Hyman, and P.W. Kincade 1993. Adv. Immunol. 54:271.

Other Notes

Small volumes of anti-CD44-SPRD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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