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Il-2-Pe Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670333

MBS670333 is an antibody targeting IL-2-PE. Also known as Rat Anti-Mouse IL-2-PE. Reactive in Mouse. Host: Rat. More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-2-PE, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse IL-2-PE
Product Synonym Names
Rat Anti-Mouse IL-2
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
JES6-5H4
Host
Rat
Species Reactivity
Mouse
Form/Format
R-phycoerythrin (R-PE) Conjugate
Specificity
Mouse Interleukin-2 (IL-2)1-3
Preparation and Storage
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Immunogen
E. coli-expressed recombinant mouse IL-2
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA and/or Flow Cytometry to conform to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-IL-2-PE antibody
ELISA
Application Notes for anti-IL-2-PE antibody
ELISA: Purified JES6-5H4 monoclonal antibody may be used as capture antibody for quantifying mouse IL-2 protein levels. It should be paired with biotinylated JES6-1A12 as detection antibody. Biotinylated is useful as a detection antibody and should be paired with purified JES6-1A12 antibody as capture antibody. ; Flow Cytometry: FITC and R-PE conjugated JES6-5H4 antibodies are useful for intracellular staining in cells producing IL-2. Intracellular staining buffers are recommended for this application.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Flow Cytometry: 10 uL/10^6 cells

Product References and Citations for anti-IL-2-PE antibody

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

Other Notes

Small volumes of anti-IL-2-PE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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