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CD4 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670336

MBS670336 is an antibody targeting CD4/L3T4C/CY7. Also known as Rat Anti-Mouse CD4/L3T4-APC/CY7. Reactive in Mouse. Host: Rat. More details

$345 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD4/L3T4C/CY7, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD4/L3T4-APC/CY7
Product Synonym Names
Rat Anti-Mouse CD4/L3T4
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
GK1.5
Host
Rat
Species Reactivity
Mouse
Form/Format
Allophycocyanin-Cyanine 7 (APC-CY 7) Conjugate
Specificity
CD4/L3T4a, Mr 52 kDa
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors.3-5 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells.1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells.7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.1,8-13
Preparation and Storage
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (RPE- CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5 Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Immunogen
Cloned mouse CTL line V41
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this package insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-CD4/L3T4C/CY7 antibody
Flow Cytometry, Immunohistochemistry (FS), Immunoprecipitation, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14
Application Notes for anti-CD4/L3T4C/CY7 antibody
Flow Cytometry: <= 0.2 ug/10^6 cells

Product References and Citations for anti-CD4/L3T4C/CY7 antibody

1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29.
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933.
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473.
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717.
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71.
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445.
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808.
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5.
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131.
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207.
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083-1091.

Other Notes

Small volumes of anti-CD4/L3T4C/CY7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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