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Cd3e-BIMA Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670344

MBS670344 is an antibody targeting CD3e-BIMA. Also known as Hamster Anti-Mouse CD3e-BIMA. Reactive in Mouse. Host: Hamster. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD3e-BIMA, Monoclonal Antibody
Full Product Name
Hamster Anti-Mouse CD3e-BIMA
Product Synonym Names
Hamster Anti-Mouse CD3epsilon
Clonality
Monoclonal
Isotype
IgG kappa
Clone Number
145-2C11
Host
Hamster
Species Reactivity
Mouse
Form/Format
Biotin-maleimide (BIMA) Conjugate
Specificity
Mouse CD3epsilon (Mr 25 kDa)
CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Preparation and Storage
The biotin (BIMA) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference. Representative data are included in this product insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-CD3e-BIMA antibody
Identification and enumeration of CD3+ cells by Flow Cytometry, Immunoprecipitation, Western Blot, In vitro depletion of CD3+ cells, In vitro activation of T cells, Immunohistochemistry
Application Notes for anti-CD3e-BIMA antibody
Flow Cytometry: <= 1 ug/10^6 cells

Product References and Citations for anti-CD3e-BIMA antibody

1. Leo, O., M. Foo, D. Sachs, L. Samuelson, and J. Bluestone. 1987. Proc. Natl. Acad. Sci. USA 84:1374.
2. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169.
3. Kruisbeek, A.M., and E. Shevach. 1991. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.12.1-3.12.14.
4. Duke, R.C., J.J. Cohen, S.A. Boehme, M.J. Lenardo, C.D. Surh, H. Kishimoto, and J. Sprent. 1995. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.17.1-3.17.33.
5. Ucker, D.S., J. Meyers, and P.D. Obermiller. 1992. J. Immunol. 149:1583.
6. Wang, R., K.M. Murphy, D.Y. Loh, C. Weaver, and J.H. Russell. 1993. J. Immunol. 150:3832.
7. Salvadori, S., B. Gansbacher, A.M. Pizzimenti, and K.S. Zier. 1994. J. Immunol. 153.5176.
8. Payer, E., A. Elbe, and G. Stingl., 1991. J. Immunol. 146:2536.

Other Notes

Small volumes of anti-CD3e-BIMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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