Rat Anti-IFNG Monoclonal Antibody – Cat# MBS670352

IFN-G (IFNG) Antibody – Rat Monoclonal

Name: IFNG Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS670352
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670352

MBS670352 is an antibody targeting Ifng. Also known as Rat Anti-Mouse IFN-g-FITC. Reactive in Mouse. Host: Rat. More details

$305 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IFN-g (Ifng), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse IFN-g-FITC

Product Gene Name

anti-Ifng antibody

Product Synonym Names

Rat Anti-Mouse IFN-gamma

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

XMG1.2

Host

Rat

Species Reactivity

Mouse

Form/Format

Fluorescein (FITC) Conjugate

Specificity

Mouse Interferon-gamma (IFN-gamma) This is a neutralizing antibody

Preparation and Storage

The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.

Molecular Weight

17,907 Da

Chromosome Location

Chromosome: 10; NC_000076.6 (118441047..118445892). Location: 10 D2; 10 66.75 cM

3D Structure

ModBase 3D Structure for P01580

Immunogen

E. coli-expressed recombinant mouse IFN-gamma

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Warning

Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Applications Tested/Suitable for anti-Ifng antibody

ELISA

Application Notes for anti-Ifng antibody

ELISA: XMG1.2 monoclonal antibody can be used as a capture antibody in a sandwich ELISA for quantifying mouse IFN-gamma protein levels. Biotinylated XMG1.2 antibody can be paired with a suitable purified capture antibody, with purified recombinant human IFN-gamma as the standard.; Western Blot: The purified XMG1.2 antibody is also useful for Western Blot applications. For Western Blot, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, has been found to enable detection of <= 100 ng/lane of recombinant mouse IFN-gamma under reducing conditions.; Immunofluorescence/Flow Cytometry: XMG1.2 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN-gamma-positive cells within mixed cell populations.4-6 Recombinant mouse IFN-gamma or Rat IgG1 should be used as a specificity control. FITC conjugated XMG1.2 antibodies are useful for intracellular staining in cells producing IFN-gamma. Intracellular staining buffers are recommended for this application.; Neutralization: The LE/AF form of XMG1.2 can be used for neutralizing mouse IFN-gamma bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-Ifng antibody

1. Cherwinski, H.M., J.H. Schumacher, K.D. Brown, and T.R. Mossman. 1987. J. Exp. Med. 166:1229.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
3. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
4. Vickingson, A., K. Pederson, and D. Muller. 1994. J. Immunol. Meth. 173:219.
5. Ferrick, D.A., M.D. Schrenzel, T. Mulvania, B. Hsieh, W.G. Ferlin, and H. Lepper. 1995. Nature 373:255.
6. Hsieh, B., M. Schrenzel, T. Mulvania, H. Lepper, L. DiMolfetto-Landon, and D. Ferrick. 1996. J. Immunol. 156:232.

Other Notes

Small volumes of anti-Ifng antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Ifng. It may not necessarily be applicable to this product.

NCBI GI #

33468859

NCBI GeneID

15978

NCBI Accession #

NP_032363.1

UniProt Primary Accession #

P01580

UniProt Secondary Accession #

Q542B8

UniProt Related Accession #

P01580

GenBank Nucleotide #

NM_008337.3

NCBI Official Full Name

interferon gamma

NCBI Official Synonym Full Names

interferon gamma

NCBI Official Symbol

Ifng

NCBI Official Synonym Symbols

Ifg; IFN-g

UniProt Protein Name

Interferon gamma

Protein Family

Interferon

UniProt Gene Name

Ifng

UniProt Synonym Gene Names

IFN-gamma

UniProt Entry Name

IFNG_MOUSE

NCBI Protein Information

interferon gamma; IFN-gamma; gamma interferon

NCBI Summary for Ifng

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

UniProt Comments for Ifng

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Released primarily from activated T lymphocytes.

Sequence similarities: Belongs to the type II (or gamma) interferon family.

Research Articles on Ifng

1. Styrax japonica glycoprotein strongly increased cytokine IFN-gamma expression in splenocytes induced by diethylnitrosamine (DEN), suggesting that glycoprotein could improve cellular immune function in DEN-induced mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ifng antibody

Reference Product	PubMed Publications
Il4 antibody	>69 publications with Ifng and Il4
Il6 antibody	>63 publications with Ifng and Il6
Il2 antibody	>38 publications with Ifng and Il2
Stat1 antibody	>28 publications with Ifng and Stat1
Ifngr1 antibody	>24 publications with Ifng and Ifngr1
Il18 antibody	>20 publications with Ifng and Il18
Cxcl9 antibody	>8 publications with Ifng and Cxcl9
Socs1 antibody	>8 publications with Ifng and Socs1
Jak2 antibody	>3 publications with Ifng and Jak2
Jak1 antibody	>3 publications with Ifng and Jak1

Pathways associated with anti-Ifng antibody

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-Ifng antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>107 publications with Ifng and Necrosis
Autoimmune Diseases Antibodies	>71 publications with Ifng and Autoimmune Diseases
Respiratory Tract Diseases Antibodies	>51 publications with Ifng and Respiratory Tract Diseases
Hepatitis Antibodies	>24 publications with Ifng and Hepatitis
MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO Antibodies	>21 publications with Ifng and MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO
Lymphoproliferative Disorders Antibodies	>16 publications with Ifng and Lymphoproliferative Disorders
Diabetes Mellitus, Type 1 Antibodies	>15 publications with Ifng and Diabetes Mellitus, Type 1
HIV Infections Antibodies	>13 publications with Ifng and HIV Infections
Arthritis, Rheumatoid Antibodies	>11 publications with Ifng and Arthritis, Rheumatoid
Hepatitis C Antibodies	>10 publications with Ifng and Hepatitis C

Organs/Tissues associated with anti-Ifng antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>32 publications with Ifng and Spleen
Mixed Antibodies	>11 publications with Ifng and Mixed
Embryonic Tissue Antibodies	>7 publications with Ifng and Embryonic Tissue