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Human IgG1 Kappa-Le/Af Antibody – Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670358

MBS670358 is an antibody targeting Human IgG1 Kappa-LE/AF. Host: Myeloma serum. More details

$255 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Human IgG1 Kappa-LE/AF, Monoclonal Antibody
Full Product Name
Human IgG1 Kappa-LE/AF
Product Synonym Names
Human IgG1kappa Isotype Control
Clonality
Monoclonal
Isotype
IgG1 kappa
Host
Myeloma serum
Form/Format
Low Endotoxin, Azide-Free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Purity/Purification
Affinity chromatography on protein G covalently linked to agarose. Purity: >95% as judged by SDS-polyacrylamide gel electrophoresis.
Preparation and Storage
Aliquot and store at or below -20°C.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for anti-Human IgG1 Kappa-LE/AF antibody
ELISA, FLISA
Application Notes for anti-Human IgG1 Kappa-LE/AF antibody
ELISA
Purified (UNLB) antibody: <=2 ug/mL
BIMA conjugate: 1:5,000 – 1:20,000
AP and HRP conjugates: 1:500 – 1:2,000

FLISA: PE conjugate: <= 1 ug/mL

Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

ELISA (EIA) of anti-Human IgG1 Kappa-LE/AF antibody

ELISA plate was coated with serially diluted Human IgG1 Kappa-UNLB (MBS679195). Immunoglobulin was detected with Mouse Anti-Human IgG1 Hinge-BIOT (MBS674029), Mouse Anti-Human IgG2 Fc-BIOT (MBS674034), Mouse Anti-Human IgG3 Hinge-BIOT (MBS674085), Mouse Anti-Human IgG4 pFc’-BIOT (MBS674073), Mouse Anti-Human IgA1-BIOT (MBS674051), Mouse Anti-Human IgA2-BIOT (MBS674057), Mouse Anti-Human IgD-BIOT (MBS674008), Mouse Anti-Human IgE Fc-BIOT (MBS674062), Mouse Anti-Human IgM-BIOT (MBS673991), Mouse Anti-Human Kappa-BIOT (MBS674091), and Mouse Anti-Human Lambda-BIOT (MBS674067) followed by Streptavidin-HRP and quantified.
Anti-Human IgG1 Kappa-Le/Af Monoclonal Antibody – Cat# MBS670358

Other Notes

Small volumes of anti-Human IgG1 Kappa-LE/AF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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