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IL-5 Antibody – Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670607

MBS670607 is an antibody targeting IL-5. Also known as Rat Anti-Human IL-5-LE/AF. Host: Rat. More details

$405 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-5, Antibody
Full Product Name
Rat Anti-Human IL-5-LE/AF
Product Gene Name
Product Synonym Names
Interleukin-5; eosinophil differentiation factor; EDF; T cell replacing factor-1; TRF-1; eosinophil colony stimulating factor; Eo-CSF; B cell growth factor-2; BCGF-2; B cell differentiation factor for IgM; BCDF-m; IgA enhancing factor; IgA-EF
Isotype
IgG2akappa
Clone Number
JES1-39D10
Host
Rat
Concentration
0.5 mg/mL (lot specific)
Specificity
Human IL-5
Preparation and Storage
Store at 2-8 degree C
Molecular Weight
15,207 Da
Immunogen
COS-expressed human IL-5
Buffer Formulation
Supplied in PBS
Isotype Control
Mouse IgG2a-LE/AF
Applications Tested/Suitable for anti-IL-5 antibody
ELISA Capture (EIA), ELISA Detection (EIA), ELISpot Capture (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC) (ICC), Neutralization
Application Notes for anti-IL-5 antibody
ELISA-Capture - Quality tested; ELISA-Detection - Quality tested; ELISpot-Capture - Reported in literature; Flow Cytometry - Reported in literature; Immunohistochemistry-Frozen Sections - Reported in literature; Immunocytochemistry - Reported in literature; Neutralization - Reported in literature

Testing Data of anti-IL-5 antibody

Rat Anti-IL-5 Antibody – Cat# MBS670607

Product References and Citations for anti-IL-5 antibody

1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcek Dekker; 1994. p. 133-57. (Immunogen, ELISA-Capture, Neut)
2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Capture)
3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture, ELISPOT-Capture)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
6. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
7. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Capture)
8. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
9. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-gamma: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (FC, ICC)
10. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet. 2000;355:1680-3. (FC)
11. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
12. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
13. Denburg JA, Silver JE, Abrams JS. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood. 1991;77:1462-8. (Neut)

Other Notes

Small volumes of anti-IL-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
Il5
UniProt Protein Name
Interleukin-5
UniProt Synonym Protein Names
B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF
UniProt Gene Name
Il5
UniProt Synonym Gene Names
Il-5; IL-5; BCGF-II; TRF
UniProt Entry Name
IL5_RAT
NCBI Protein Information
interleukin-5

NCBI Summary for IL-5

cytokine with B-cell growth factor activity [RGD, Feb 2006]

UniProt Comments for IL-5

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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