• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD2 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673127

MBS673127 is an antibody targeting Cd2. Also known as Rat Anti-Mouse CD2/LFA-2-LE/AF. Reactive in Mouse. Host: Rat. More details

$415 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD2 (LFA-2), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD2/LFA-2-LE/AF
Product Gene Name
anti-Cd2 antibody
Product Synonym Names
Rat Anti-Mouse CD2/LFA-2 - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD2 (LFA-2)
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
12-15
Host
Rat
Species Reactivity
Mouse
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Specificity
Mouse CD2/LFA-2 (Mr 45-58 kDa)
CD2, also known as leukocyte function-associated antigen-2 (LFA-2), is a member of the immunoglobulin superfamily of cell adhesion molecules.^1 It is expressed on the surface of >95% of thymocytes, and essentially all B cells, T cells and NK cells. ^1-3 In the mouse, CD2 is the receptor for CD48.^4 CD2 is thought to play a major role in mediating T-cell adhesion, T-cell recognition/activation, Tcell signaling and intrathymic differentiation.^5-10
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Molecular Weight
38,415 Da
Chromosome Location
Chromosome: 3; NC_000069.5 (101079831..101091862, complement). Location: 3 F2.2; 3 48.2 cM
3D Structure
ModBase 3D Structure for P08920
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent. Representative data are included in this data sheet.
Applications Tested/Suitable for anti-Cd2 antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-Cd2 antibody
Research Application(s): In vitro inhibition of CTL-mediated lysis
Induction of T cell unresponsiveness in vivo

Product References and Citations for anti-Cd2 antibody

1. Altevogt, P., U. Kohl, P. von Hoegen, E. Lang, and V. Schirrmacher. 1989. Eur. J. Immunol. 19:341-346.
2. Altevogt, P., M. Michaelis, and R.A. and B. A. Kyewski. 1989. Eur. J. Immunol. 19:1509.
3. Kato, K., M. Koyanagi, H. Okada, T. Takanashi, Y.W. Wong, A.F. Williams, K. Okumura, and H. Yagita. 1992. J. Exp. Med. 176:1241-1249.
4. Guckel, B., C. Berek, M. Lutz, P. Altevogt, V. Schirrmacher, and B.A. Kyewski. 1991. J. Exp. Med. 174:947.
5. Shaw, S., G.E.G. Luce, R. Quinones, R.E. Gress, T.A. Springer, and M.E. Sanders. 1986. Nature (London)323:262-264.
6. Shaw, S., and G.E.G. Luce. 1987. J. Immunol. 139:1037-1045.
7. Bierer, B.E., A. Peterseon, J. Barbosa, B. Seed, and S.J. Burakoff. 1988. Proc. Natl. Acad. Sci. USA 85:1194-1198.
8. Alcover, A., D. Ramarli, N.E. Richardson, H. Chang, and E.L. Reinherz. Immunol. Rev. 95:5-36.
9. Linch, D.C., D.L. Wallace, and K. O'Flynn. 1987. Immunol. Rev. 95:137-159.
10. Selvaraj, O., M.L. Plunkett, M.Dustin, M.E. Sanders, S. Shaw, and T.A. Springer. Nature (London) 326:400-403.

Other Notes

Small volumes of anti-Cd2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd2. It may not necessarily be applicable to this product.

NCBI GI #
31657218
NCBI GeneID
12481
UniProt Primary Accession #
P08920
UniProt Secondary Accession #
Q61394
UniProt Related Accession #
P08920; Q3UU70; Q9R201
NCBI Official Full Name
CD2 antigen
NCBI Official Synonym Full Names
CD2 antigen
NCBI Official Symbol
Cd2
NCBI Official Synonym Symbols
Ly37; LFA-2; Ly-37
UniProt Protein Name
T-cell surface antigen CD2
UniProt Synonym Protein Names
LFA-2; LFA-3 receptor; Lymphocyte antigen 37; Ly-37; T-cell surface antigen T11/Leu-5
Protein Family
T-cell surface antigen
UniProt Gene Name
Cd2
UniProt Synonym Gene Names
Ly-37
UniProt Entry Name
CD2_MOUSE
NCBI Protein Information
T-cell surface antigen CD2; LFA-3 receptor; lymphocyte antigen 37; T-cell surface antigen T11/Leu-5

UniProt Comments for Cd2

Function: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Subunit structure: Interacts with CD2AP and PSTPIP1. Ref.6 Ref.7

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Show more

Research Articles on Cd2

1. CD244 inhibition and activation depends on CD2 and phospholipase C-gamma1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cd2 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-Cd2 antibody

 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >744 publications with Cd2 and Lymphoma
 Lymphoma, B-Cell Antibodies  >215 publications with Cd2 and Lymphoma, B-Cell
 Inflammation Antibodies  >209 publications with Cd2 and Inflammation
 Chromosome Aberrations Antibodies  >156 publications with Cd2 and Chromosome Aberrations
 Kidney Diseases Antibodies  >132 publications with Cd2 and Kidney Diseases
 Liver Diseases Antibodies  >116 publications with Cd2 and Liver Diseases
 Skin Neoplasms Antibodies  >111 publications with Cd2 and Skin Neoplasms
 Cell Transformation, Neoplastic Antibodies  >89 publications with Cd2 and Cell Transformation, Neoplastic
 Hyperplasia Antibodies  >48 publications with Cd2 and Hyperplasia
 Hepatitis Antibodies  >47 publications with Cd2 and Hepatitis

Organs/Tissues associated with anti-Cd2 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >656 publications with Cd2 and Bone Marrow
 Kidney Antibodies  >443 publications with Cd2 and Kidney
 Thymus Antibodies  >427 publications with Cd2 and Thymus
 Spleen Antibodies  >262 publications with Cd2 and Spleen
 Mammary Gland Antibodies  >15 publications with Cd2 and Mammary Gland
 Uncharacterized Tissue Antibodies  >4 publications with Cd2 and Uncharacterized Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000