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CD4 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673164

MBS673164 is an antibody targeting Cd4. Also known as Rat Anti-Mouse CD4/L3T4-CY5. Reactive in Mouse. Host: Rat. More details

$400 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD4/L3T4, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD4/L3T4-CY5
Product Gene Name
anti-Cd4 antibody
Product Synonym Names
Rat Anti-Mouse CD4/L3T4 - Cyanine5-Labeled; Rat Anti-Mouse CD4/L3T4
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2b kappa
Clone Number
GK1.5
Host
Rat
Species Reactivity
Mouse
Form/Format
Cyanine 5 (CY5) Conjugate
Specificity
CD4/L3T4a, Mr 52 kDa
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.^1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. ^3-6 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. ^1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4^+ cells.^7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.^1,8-13
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (RPE- CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5 Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Molecular Weight
51,297 Da
Chromosome Location
Chromosome: 6; NC_000072.5 (124814711..124838227, complement). Location: 6 F2; 6 60.18 cM
3D Structure
ModBase 3D Structure for P06332
Immunogen
Cloned mouse CTL line V4
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this package insert.
Applications Tested/Suitable for anti-Cd4 antibody
Flow Cytometry; Immunohistochemistry; Immunoprecipitation
Application Notes for anti-Cd4 antibody
Research Application(s): In vivo and in vitro depletion of CD4^+ cells
Inhibition of T helper function
Fractionation of CD4^+ cells

Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells

Product References and Citations for anti-Cd4 antibody

1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29.
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933.
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473.
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717.
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71.
6. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445.
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808.
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5.
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131.
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207.
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.
15. Jia, W. and J. F. Kearney. 1996. Personal communication.

Other Notes

Small volumes of anti-Cd4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd4. It may not necessarily be applicable to this product.

NCBI GI #
24659577
NCBI GeneID
12504
UniProt Primary Accession #
P06332
UniProt Related Accession #
O55054; P06332; P70443; Q3TSV7; Q61396
NCBI Official Full Name
CD4 antigen
NCBI Official Synonym Full Names
CD4 antigen
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
L3T4; Ly-4
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Synonym Protein Names
T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_MOUSE
NCBI Protein Information
T-cell surface glycoprotein CD4; OTTMUSP00000027399; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

UniProt Comments for Cd4

Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subunit structure: Associates with p56-LCK. Interacts with SPG21

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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Research Articles on Cd4

1. anti-TIM3 requires IFN-gamma producing CD8(+) T cells and CD4(+) T cells, and a higher ratio of tumor infiltrating CD8(+):CD4(+) T cells correlating with therapeutic success

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cd4 antibody

 Reference Product  PubMed Publications
 Runx1 antibody  >56 publications with Cd4 and Runx1

Pathways associated with anti-Cd4 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-Cd4 antibody

 Disease Name  Pubmed Publications
 Immunologic Deficiency Syndromes Antibodies  >30050 publications with Cd4 and Immunologic Deficiency Syndromes
 Neoplasms Antibodies  >12976 publications with Cd4 and Neoplasms
 Inflammation Antibodies  >8565 publications with Cd4 and Inflammation
 Respiratory Tract Diseases Antibodies  >6209 publications with Cd4 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >5362 publications with Cd4 and Lung Diseases
 Lymphoma Antibodies  >4069 publications with Cd4 and Lymphoma
 AIDS-Related Opportunistic Infections Antibodies  >3821 publications with Cd4 and AIDS-Related Opportunistic Infections
 Leukemia Antibodies  >3440 publications with Cd4 and Leukemia
 Neoplasms, Experimental Antibodies  >2480 publications with Cd4 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >2299 publications with Cd4 and Cardiovascular Diseases

Organs/Tissues associated with anti-Cd4 antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >7905 publications with Cd4 and Spleen
 Thymus Antibodies  >6440 publications with Cd4 and Thymus
 Mixed Antibodies  >3162 publications with Cd4 and Mixed
 Brain Antibodies  >2494 publications with Cd4 and Brain
 Salivary Gland Antibodies  >347 publications with Cd4 and Salivary Gland
 Mammary Gland Antibodies  >130 publications with Cd4 and Mammary Gland
 Pineal Gland Antibodies  >32 publications with Cd4 and Pineal Gland
 Uncharacterized Tissue Antibodies  >13 publications with Cd4 and Uncharacterized Tissue
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