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CD5 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673169

MBS673169 is an antibody targeting CD5/Lyt-1. Also known as Rat Anti-Mouse CD5/Ly-1-FITC. Reactive in Mouse. Host: Rat. More details

$370 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD5/Lyt-1, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD5/Ly-1-FITC
Product Synonym Names
Rat Anti-Mouse CD5/Ly-1 - FITC; Rat Anti-Mouse CD5/Lyt-1
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
B19.1
Host
Rat
Species Reactivity
Mouse
Form/Format
Fluorescein (FITC) Conjugate
Specificity
CD5/Lyt-1, Mr 67 kDa
CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells.^1-3 It has been identified as the major ligand of the B-cell antigen CD72.^4, 5 The frequency of CD5^+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.^7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.^13, 14
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference by flow cytometry. Representative data are included in this data sheet.
Applications Tested/Suitable for anti-CD5/Lyt-1 antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation; Western Blot
Application Notes for anti-CD5/Lyt-1 antibody
Research Application(s): Identification and enumeration of CD5^+ cells by flow cytometry
Identification of CD4^+ cells in acetone-fixed frozen sections
Activation of T cells in vitro

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-CD5/Lyt-1 antibody

1. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63.
2. Seaman, W. E., D. Wofsy, J. S. Greenspan, and J. A. Ledbetter. 1983. J. Immunol. 130:1713.
3. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280.
4. Van de Veld, H., I. von Hoegen, W. Luo, J. R. Parnes, and K. Thielemans. 1991. Nature 351:662.
5. Luo, W., H. Van de Velde, I. von Hoegen, J. R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630.
6. Kantor, A. B. and L. A. Herzenberg. 1993. Ann. Rev. Immunol. 11:501.
7. Holland, N. 1982. Immunol. Rev. 68:43.
8. Stanton, T., T. L. Stevens, J. A. Ledbetter, and D. Wofsy. 1986. J. Immunol. 136:1734.
9. Verwilghen, J. R., P. Vandesande, and J. L. Ceuppens. 1996. Cell Immunol. 131:109.
10. Logdberg, L. and E. M. Shevach. 1996. Eur. J. Immunol. 15:1007.
11. June, C. H., P. R. Rabinovitch, and J. A. Ledbetter. 1987. J. Immunol. 138:2782.
12. Hollander, N., E. Pillemer, and I. L. Weissman. 1981. Proc. Natl. Acad. Sci. U. S. A. 78:1148.
13. Tarakhovsky, A., S.B. Kanner, J. Hombach, J.A. ledbetter, W. Muller, N. Killeen, and K. Rajewsky. 1995. Science 269:535.
14. Bikah, G., J. Carey, J.R. Cialella, A. Tarakhovsky, and S, Bobdada. 1996. Science 274:1906.
15. Moratz, C. and J. F. Kearney. 1996. Personal communication.

Other Notes

Small volumes of anti-CD5/Lyt-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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