• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673252

MBS673252 is an antibody targeting CD19. Also known as Mouse Anti-Mouse CD19-SPRD. Reactive in Mouse. Host: Mouse. More details

$425 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD19, Monoclonal Antibody
Full Product Name
Mouse Anti-Mouse CD19-SPRD
Product Gene Name
anti-CD19 antibody
Product Synonym Names
Mouse Anti-Mouse CD19 - SpectralRed-Labeled; Mouse Anti-Mouse CD19
Clonality
Monoclonal
Isotype
Mouse (129 x C57BL/6) IgA kappa
Clone Number
MB19-1
Host
Mouse
Species Reactivity
Mouse
Form/Format
Spectral Red (SPRD) Conjugate
Specificity
CD19 B cell differentiation antigen, Mr 95 kDa
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220^+/CD43^+/HSA^+) through fully differentiated B cell stages. ^1-7 Terminally differentiated plasma cells do not express CD19. ^1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. ^3, 4 All splenic and peritoneal IgM^+ cells of both B-1 and B-2 lineages are CD19^+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. ^1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. ^7
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Molecular Weight
61,128 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (28943260..28950668). Location: 16p11.2
OMIM
107265
3D Structure
ModBase 3D Structure for P15391
Immunogen
CD19^+ mouse pre-B cell line, 300.19
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CD19 antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-CD19 antibody
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells

Product References and Citations for anti-CD19 antibody

1. Sato, S., N. Ono, D.A. Steeber, D.S. Pisetsky, and T.F. Tedder. 1996. J. Immunol. 157:4371-4378.
2. Krop, I., A.R. Fougerolles, R.R. Hardy, M. Allison, M.S. Schlissel, and D.T. Fearon. 1996. Eur. J. Immunol. 26:238.
3. Krop, I., A.L. Shaffer, D.T. Fearon, and M.S. Schlissel. 1996. J. Immunol. 157:48.
4. Fearon, D.T. 1993. Curr. Opin. in Immunol. 5:341.
5. Tedder, T.F., L.-J. Zhou, and P. Engel. 1994. Immunol. Today 15:437.
6. Rickert, R.C., K. Rajewsky, and J. Roes. 1995. Nature 376:352.
7. Engel, P., L.-J. Zhou, D.C. Ord, S. Sato, B. Koller, and T.F. Tedder. 1995. Immunity 3:39.
8. Rolink, A., E. ten Boekel, F. Melchers, D.T. Fearon, I. Krop, and J. Andersson. 1996. J. Exp. Med. 183:187.

Other Notes

Small volumes of anti-CD19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD19. It may not necessarily be applicable to this product.

NCBI GI #
901823
NCBI GeneID
930
UniProt Primary Accession #
P15391
UniProt Secondary Accession #
Q96S68; Q9BRD6; A0N0P9
UniProt Related Accession #
P15391; Q6ZNU2; Q71UW0
NCBI Official Full Name
CD19
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3; MGC12802
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Synonym Protein Names
B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
Protein Family
B-lymphocyte antigen
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN
NCBI Protein Information
B-lymphocyte antigen CD19; OTTHUMP00000122551; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4

NCBI Summary for CD19

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]
Show more

UniProt Comments for CD19

Function: Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Subunit structure: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein.

Post-translational modification: Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Ref.10 Ref.11 Ref.12

Involvement in disease: Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [

MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. Ref.13

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence AAA35533.1 differs from that shown. Reason: Frameshift at position 396.
Show more

Research Articles on CD19

1. CD23 and CD19 are important factors that associated with serum total IgE in the pathogenesis of allergic rhinitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD19 antibody

 Reference Product  PubMed Publications
 CD22 antibody  >540 publications with CD19 and CD22
 CD79A antibody  >121 publications with CD19 and CD79A
 CD79B antibody  >90 publications with CD19 and CD79B
 CD81 antibody  >84 publications with CD19 and CD81
 CD9 antibody  >75 publications with CD19 and CD9
 CR2 antibody  >72 publications with CD19 and CR2
 LYN antibody  >53 publications with CD19 and LYN
 SYK antibody  >38 publications with CD19 and SYK
 BTK antibody  >35 publications with CD19 and BTK
 VAV1 antibody  >13 publications with CD19 and VAV1

Pathways associated with anti-CD19 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram

Diseases associated with anti-CD19 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2577 publications with CD19 and Neoplasms
 Lung Diseases Antibodies  >167 publications with CD19 and Lung Diseases
 Hypersensitivity Antibodies  >146 publications with CD19 and Hypersensitivity
 Arthritis, Rheumatoid Antibodies  >145 publications with CD19 and Arthritis, Rheumatoid
 Agammaglobulinemia Antibodies  >72 publications with CD19 and Agammaglobulinemia
 Common Variable Immunodeficiency Antibodies  >67 publications with CD19 and Common Variable Immunodeficiency
 Asthma Antibodies  >61 publications with CD19 and Asthma
 Pneumonia Antibodies  >58 publications with CD19 and Pneumonia
 Blood Coagulation Disorders Antibodies  >44 publications with CD19 and Blood Coagulation Disorders
 Liver Neoplasms Antibodies  >41 publications with CD19 and Liver Neoplasms

Organs/Tissues associated with anti-CD19 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5063 publications with CD19 and Blood
 Bone Marrow Antibodies  >1451 publications with CD19 and Bone Marrow
 Spleen Antibodies  >439 publications with CD19 and Spleen
 Lymph Node Antibodies  >387 publications with CD19 and Lymph Node
 Lymph Antibodies  >343 publications with CD19 and Lymph
 Lung Antibodies  >171 publications with CD19 and Lung
 Thymus Antibodies  >152 publications with CD19 and Thymus
 Tonsil Antibodies  >104 publications with CD19 and Tonsil
 Brain Antibodies  >86 publications with CD19 and Brain
 Intestine Antibodies  >84 publications with CD19 and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000