CD24 Antibody – Rat Monoclonal

Monoclonal

Rat IgG2a kappa

91

Rat

Mouse

Fluorescein (FITC) Conjugate

Mouse heat stable antigen (HSA); Mr 35^-45 kDa
CD24, also known as heat stable antigen, is a phosphatidylinositol^-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes.^1^-3 The absence of expression from mature T cells is closely associated with their maturation from CD4^+CD8^+CD24^+ thymocytes to either CD4^+CD8^-CD24^- or CD4^-CD8^+CD24^- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. ^4^-6 Its ligand is P^-selectin (CD62P). ^7 CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. ^1^-3 The monoclonal antibody 91 can be used to inhibit B^-cell differentiation. ^8

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this data sheet.