• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD25 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673295

MBS673295 is an antibody targeting CD25/IL-2R alpha. Also known as Rat Anti-Mouse CD25/IL-2Ra-LE/AF. Reactive in Mouse. Host: Rat. More details

$415 / 0.5 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD25/IL-2R alpha, Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD25/IL-2Ra-LE/AF
Product Synonym Names
Rat Anti-Mouse CD25/IL-2Ra - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD25/IL-2R alpha
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
3C7
Host
Rat
Species Reactivity
Mouse
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Specificity
Low affinity alpha chain IL-2 receptor, Mr 55 kDa (IL-2R alpha)
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.^4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.^5 It is expressed at a higher level on CD4^+CD8^+ thymocytes.^5, 6 It is also expressed on cultured epidermal Langerhans cells.^7 The biochemical features of murine CD25 have been characterized in detail.^8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.^2, 11, 12 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.^2, 13
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CD25/IL-2R alpha antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-CD25/IL-2R alpha antibody
Research Application(s): Identification and enumeration of CD25^+ cells by flow cytometry
Inhibition of IL-2 or mitogen-induced T cell proliferation
In vitro blocking of IL-2 binding

Product References and Citations for anti-CD25/IL-2R alpha antibody

1. Malek, T. R., R. J. Robb, and E. M. Shevach. 1983. Proc. Natl. Acad. Sci. U. S. A. 80:5694.
2. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970.
3. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405.
4. Andrew, M. E., A. M. Churilla, T. R. Malek, V. L. Braciale, and T. J. Braciale. 1985. J. Immunol. 134:920.
5. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265.
6. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456.
7. Steiner, G., E. Tschachler, M. Tani, T. R. Malek, E. M. Shevach, W. Holter, W. Knapp, K. Wolff, and G. Stingl. 1986. J. Immunol. 137:155.
8. Malek, T. R. and P. E. Korty. 1986. J. Immunol. 136:4092.
9. Malek, T. R., J. D. Ashwell, R. N. Germain, E. M. Shevach, and J. Miller. 1986. Immunol. Rev. 92:81.
10. Saragovi, H. and T. R. Malek. 1988. J. Immunol. 141:476.
11. Lorenzo, F., C. Jaulin, N. Vita, P. Froussard, P. Ferrara, D. L. Jankovic, and J. Theze. 1991. J. Immunol. 147:2970.
12. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929.
13. Malek, T. R., G. Ortega, J. P. Jakway, C. Chan, and E. M. Shevach. 1984. J. Immunol. 133:1976.
14. Leonard, W.J., et. al. 1984. Nature 311:626
15. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257

Other Notes

Small volumes of anti-CD25/IL-2R alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000