CD16 Antibody – Rat Monoclonal

Monoclonal

Rat IgG2a

93

Rat

Mouse

Low Endotoxin, Azide - Free (LE/AF)

Murine CD16/32 (CD16/Fc gamma II and CD32/Fc gamma III receptors)
The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.^1, 2 The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.^1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.^4 Besides identifying Fc gamma R^+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.^4-6 The monoclonal antibody 93 recognizes a conformational epitope formed by Fc gamma II and Fc gamma III receptors.

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

To insure lot-to-lot consistency, each batch of product is tested by flow cytometry for conformance with characteristics of a standard reference reagent.