Cd49e/VLA-5 Antibody – Rat Monoclonal

Monoclonal

Rat (Lewis) IgG2a kappa

5H10-27 (MFR5)

Rat

Mouse

Biotin (BIOT) Conjugate

alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.^1-5 Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro.^1, 2-4, 6 Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8^+ T cells to plate-bound anti- CD3 monoclonal antibody.^3

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

C57BL/6 x A/J F1 mouse mast cell line MC/9 1

To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.