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CD102 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673723

The 3C4 monoclonal antibody reacts with the cell surface glycoprotein mouse CD102 (ICAM-2), a ligand for the leucocyte integrin CD11a/CD18 (LFA-1). CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells. It is… More details

$355 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD102 (ICAM-2), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD102/ICAM-2-UNLB
Product Synonym Names
Rat Anti-Mouse CD102/ICAM-2 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD102 (ICAM-2); ICAM-2, intercellular adhesion molecule-2
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
3C4 (mlC2/4)
Host
Rat
Species Reactivity
Mouse
Form/Format
UNLB (Unconjugated)
Concentration
0.5 mg/mL (lot specific)
Specificity
Mouse CD102; Mr 55 kDa
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8°C.
Immunogen
COS cells transfected with mouse ICAM-2 cDNA and BW5147 AKR/J mouse thymoma cell line
Buffer
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Applications Tested/Suitable for anti-CD102 (ICAM-2) antibody
Flow Cytometry – Quality tested 1,5
Immunohistochemistry-Frozen Sections – Reported in literature 2,3
Immunocytochemistry – Reported in literature 4,5
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1
Application Notes for anti-CD102 (ICAM-2) antibody
Flow Cytometry: Purified (UNLB) antibody <= 1ug/106 cells
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.


References:
1. Xu H, Bickford JK, Luther E, Carpenito C, Takei F, Springer TA. Characterization of murine intercellular adhesion molecule-2. J Immunol. 1996;156:4909-14. (Immunogen, FC, IP, Block)
2. Cao G, Savani RC, Fehrenbach M, Lyons C, Zhang L, Coukos G, et al. Involvement of endothelial CD44 during in vivo angiogenesis. Am J Pathol. 2006;169:325-36. (IHC-FS)
3. Cao G, Fehrenbach ML, Williams JT, Finklestein JM, Zhu J, DeLisser HM. Angiogenesis in platelet endothelial cell adhesion molecule-1-null mice. Am J Pathol. 2009;175:903-15. (IHC-FS)
4. Shin J, Lee JC, Baek K. A single extra copy of Dscr1 improves survival of mice developing spontaneous lung tumors through suppression of tumor angiogenesis. Cancer Lett. 2014;342:70-81. (ICC)
5. Fehrenbach ML, Cao G, Williams JT, Finklestein JM, DeLisser HM. Isolation of murine lung endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2009;296:L1096-1103. (ICC, FC)

Testing Data of anti-CD102 (ICAM-2) antibody

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD102-PE (MBS673727) and Rat-Anti-Mouse CD19-FITC (MBS673236)
Rat Anti-CD102 Monoclonal Antibody – Cat# MBS673723

Product References and Citations for anti-CD102 (ICAM-2) antibody

1. Xu, H., J.K. Bickford, E. Luther, C. Carpenito, F. Takei, and T.A. Springer. 1996. J. Immunol. 156:4909.
2. Xu, H., O.L. Tong, A.R. deFougerolles, and T.A. Springer. 1992. J. Immunol. 149:2650.

Other Notes

Small volumes of anti-CD102 (ICAM-2) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD102 (ICAM-2) purchase

MBS673723 is an antibody targeting CD102 (ICAM-2). Also known as Rat Anti-Mouse CD102/ICAM-2-UNLB. Reactive in Mouse. Host: Rat.
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