Mouse Anti-CD21 Monoclonal Antibody – Cat# MBS673826

CD21 Antibody – Mouse Monoclonal

Name: CD21 Antibody; Monoclonal; Mouse; Porcine-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS673826
Price: 570 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673826

MBS673826 is an antibody targeting CD21. Also known as Mouse Anti-Porcine CD21-FITC. Reactive in Porcine. Host: Mouse. More details

$570 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD21, Monoclonal Antibody

Full Product Name

Mouse Anti-Porcine CD21-FITC

Product Synonym Names

Mouse Anti-Porcine CD21 - FITC; Mouse Anti-Pig CD21

Clonality

Monoclonal

Isotype

Mouse (BALB/c) IgG1 kappa

Clone Number

BB6-11C9.6

Host

Mouse

Species Reactivity

Porcine

Form/Format

Fluorescein (FITC) Conjugate

Specificity

Porcine CD21, Mr 160 kDa
CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells.1 Low density CD21 has also been reported on granulocytes and monocytes. MAb BB6-11C9.6 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer's patches (ILPP); 93-97% in spleen; and 0% in thymus.2 The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation).2

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Characterization

To ensure optimal performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-CD21 antibody

Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation

Application Notes for anti-CD21 antibody

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-CD21 antibody

1. Tedder T.F., L.T. Clement, and Cooper M.D.. Expression of C3d receptors during human B cell differentiation: Immunofluorescence analysis with the HB-5 monoclonal antibody. J Immunol. 1984:133:678.
2. Denham, S., R.J. Zwart, J.T.D. Whittall, M. Pampusch, A.H. Corteyn, A.T.J. Bianchi, M.P. Murtaugh, R.M.E. Parkhouse, H. Tlaskalova, J. Sinkora, M. Siinkora, and Z. Rehakova. 1998. Monoclonal antibodies putatively identifying porcine B cells. Veter. Immunol. Immunopathol. 60:317-328.
3. Pescovitz, M. Personal communication.

Other Notes

Small volumes of anti-CD21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD21. It may not necessarily be applicable to this product.

Protein Family

Complement receptor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.