Gamma Delta (TCR) Antibody – Mouse Monoclonal

Monoclonal

Mouse IgG1 kappa

TCR1

Mouse

Chicken

Fluorescein (FITC) Conjugate

Chicken TCR gamma delta
MAb TCR1 precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates. Unlike human and mouse, in which gamma delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma delta T cells.1-3 The frequency of TCR gamma delta cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCR gamma delta + cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCR gamma delta + cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma delta T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.5

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan flow cytometer.