Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS673894

CD28 Antibody – Mouse Monoclonal

Name: CD28 Antibody; Monoclonal; Mouse; Chicken-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS673894
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673894

MBS673894 is an antibody targeting Cd28. Also known as Mouse Anti-Chicken CD28-BIOT. Reactive in Chicken. Host: Mouse. More details

$480 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD28, Monoclonal Antibody

Full Product Name

Mouse Anti-Chicken CD28-BIOT

Product Gene Name

anti-Cd28 antibody

Product Synonym Names

Mouse Anti-Chicken CD28 - Biotin; Mouse Anti-Chicken CD28

Clonality

Monoclonal

Isotype

Mouse IgG1 kappa

Clone Number

AV7

Host

Mouse

Species Reactivity

Chicken

Form/Format

Biotin (BIOT) Conjugate

Specificity

Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28
Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma delta + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V beta 1- and V beta 2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2 MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.3

Preparation and Storage

The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added; store at 2-8 degree C.
The FITC conjugate is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1% sodium azide as preservative (PBS/NaN3); store at 2-8 degree C.
The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2-8 degree C.
The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 degree C.
All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chromosome Location

Chromosome: 1; NC_000067.5 (60803232..60830203). Location: 1 C1-C3; 1 30.1 cM

3D Structure

ModBase 3D Structure for P31041

Characterization

To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this data sheet.

Applications Tested/Suitable for anti-Cd28 antibody

Flow Cytometry; Immunoprecipitation

Application Notes for anti-Cd28 antibody

Research Application(s): In vitro T-cell costimulation

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-Cd28 antibody

1. Young, J.R., T.F. Davison, C.A. Tregaskes, M.C. Rennie, and O. Vainio. 1994. J. Immunol. 152:3848.
2. Arstila, T.P., O. Vainio, and O. Lassila. 1994. Scand J. Immunol. 40:368.

Other Notes

Small volumes of anti-Cd28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cd28. It may not necessarily be applicable to this product.

NCBI GI #

192485

NCBI GeneID

12487

UniProt Primary Accession #

P31041

UniProt Related Accession #

P31041; Q8CDB3

NCBI Official Full Name

CD28

NCBI Official Synonym Full Names

CD28 antigen

NCBI Official Symbol

Cd28

NCBI Official Synonym Symbols

Cd28

UniProt Protein Name

T-cell-specific surface glycoprotein CD28

Protein Family

T-cell-specific surface glycoprotein

UniProt Entry Name

CD28_MOUSE

NCBI Protein Information

T-cell-specific surface glycoprotein CD28; OTTMUSP00000002157

UniProt Comments for Cd28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: membrane; integral to membrane; plasma membrane; immunological synapse; lipid raft; external side of plasma membrane

Molecular Function: protein homodimerization activity; SH3/SH2 adaptor activity; protease binding

Biological Process: positive regulation of isotype switching to IgG isotypes; T cell activation; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of inflammatory response to antigenic stimulus; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation; immune response; positive regulation of T cell proliferation; regulatory T cell differentiation

Research Articles on Cd28

1. blocking the CD28/B7 signal transduction pathway with lentiviral vector-mediated RNA interference effectively controlled the occurrence of mouse GVHD following allogeneic bone marrow transplantation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cd28 antibody

Products by Pathway	Pathway Diagram
Adaptive Immunity Signaling Pathway antibodies	Adaptive Immunity Signaling Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
CD28 Co-stimulation Pathway antibodies	CD28 Co-stimulation Pathway Diagram
CD28 Dependent PI3K/Akt Signaling Pathway antibodies	CD28 Dependent PI3K/Akt Signaling Pathway Diagram
CD28 Dependent Vav1 Pathway antibodies	CD28 Dependent Vav1 Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram

Organs/Tissues associated with anti-Cd28 antibody

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>487 publications with Cd28 and Spleen
Bone Antibodies	>329 publications with Cd28 and Bone
Bone Marrow Antibodies	>298 publications with Cd28 and Bone Marrow
Thymus Antibodies	>296 publications with Cd28 and Thymus
Skin Antibodies	>266 publications with Cd28 and Skin
Lung Antibodies	>261 publications with Cd28 and Lung
Joint Antibodies	>116 publications with Cd28 and Joint
Embryonic Tissue Antibodies	>50 publications with Cd28 and Embryonic Tissue
Mammary Gland Antibodies	>3 publications with Cd28 and Mammary Gland