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Cht1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673979

MBS673979 is an antibody targeting ChT1. Also known as Mouse Anti-Chicken ChT1-UNLB. Reactive in Chicken. Host: Mouse. More details

$395 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ChT1, Monoclonal Antibody
Full Product Name
Mouse Anti-Chicken ChT1-UNLB
Product Synonym Names
Mouse Anti-Chicken ChT1 - Purified, Unlabeled Antibody; Mouse Anti-Chicken ChT1
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
CT1
Host
Mouse
Species Reactivity
Chicken
Form/Format
Purified (UNLB) Antibody
Specificity
ChT1 antigen (Mr 63/104 kDa) on chicken thymocytes1
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function.3 CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers.4 CT1 also recognizes quail cortical thymocytes.1
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Immunogen
Chicken thymocytes
Characterization
To ensure optimal performance, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative flow data are shown on page 2 of this product insert.
Applications Tested/Suitable for anti-ChT1 antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-ChT1 antibody
Research Application(s): Identification and enumeration of ChT1+ cells by flow cytometry
In vitro inhibition of T cell differentiation

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-ChT1 antibody

1. Chen, C.L., H.T.C. Chanh, and M.D. Cooper. 1984. Chicken thymocyte-specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization. Eur. J. Immunol. 14:385.
2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.
3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.
4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.

Other Notes

Small volumes of anti-ChT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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