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Cd11b (Mac-1 Alpha, Integrin Alpha M) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674201

MBS674201 is an antibody targeting CD11b (Mac-1 alpha, Integrin alpha M). Also known as Mouse Anti-Human CD11b/Mac-1a-SPRD. Reactive in Human. Host: Mouse. More details

$420 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11b (Mac-1 alpha, Integrin alpha M), Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD11b/Mac-1a-SPRD
Product Synonym Names
Mouse Anti-Human CD11b/Mac-1a - SpectralRed-Labeled; Mouse Anti-Human CD11b (Mac-1 alpha, Integrin alpha M)
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
ICRF44
Host
Mouse
Species Reactivity
Human
Form/Format
Spectral Red (SPRD) Conjugate
Specificity
Human CD11b/Mac-1 alpha
CD11b is the 165 kDa alpha M chain which combines with the CD18 beta 2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b/Mac-1 alpha functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2).1-8
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of phosphate buffered saline containing 0.1% PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Flow Cytometry; Immunohistochemistry (Frozen Sections)
Research Application(s): Blocking Studies

Flow Cytometry Suggested Dilution: 10 uL/10^6 cells

Product References and Citations for anti-CD11b (Mac-1 alpha, Integrin alpha M) antibody

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Springer, T.A. 1994. Cell 76:301.
4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD11b Section, Academic Press, New York, p. 158.
5. Lee, J.O., L.A. Bankston, M.A. Arnaout, and R.C. Liddington. 1995. Structure 3:1333.
6. Zhang, L.., and E.F. Plow. 1996. J. Biol. Chem. 271:18211.
7. Davis, G.E. 1992. Exp. Cell. Res. 200:242.
8. Perry, H.R., and R.F. Todd, III. 1996. Immunol. Today 17:209.

Other Notes

Small volumes of anti-CD11b (Mac-1 alpha, Integrin alpha M) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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