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CD16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674228

MBS674228 is an antibody targeting CD16 (Fc gamma RIII). Also known as Mouse Anti-Human CD16-FITC. Reactive in Human. Host: Mouse. More details

$335 / 100 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD16 (Fc gamma RIII), Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD16-FITC
Product Synonym Names
Mouse Anti-Human CD16 - FITC; Mouse Anti-Human CD16 (Fc gamma RIII)
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
GRM1
Host
Mouse
Species Reactivity
Human
Form/Format
Fluorescein (FITC) Conjugate
Specificity
Human CD16/Fc gamma RIII
CD16, a member of the immunoglobulin superfamily, is a 50-65 kDa glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction.1-5
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CD16 (Fc gamma RIII) antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells

Product References and Citations for anti-CD16 (Fc gamma RIII) antibody

1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD16 Section, Academic Press, New York, p. 173.
4. Ravetch, J.V., and B. Perussia. 1989. J. Exp. Med. 170:481.
5. Takai, T., M. Li, D. Sylvestre, R. Clynes, and J.V. Ravetch. 1994. Cell 76:519.

Other Notes

Small volumes of anti-CD16 (Fc gamma RIII) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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