Mouse Anti-CD33 Monoclonal Antibody – Cat# MBS674236

CD33 Antibody – Mouse Monoclonal

Name: CD33 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS674236
Price: 395 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674236

MBS674236 is an antibody targeting CD33. Also known as Mouse Anti-Human CD33-SPRD. Reactive in Human. Host: Mouse. More details

$395 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD33, Monoclonal Antibody

Full Product Name

Mouse Anti-Human CD33-SPRD

Product Gene Name

anti-CD33 antibody

Product Synonym Names

Mouse Anti-Human CD33 - SpectralRed-Labeled; Mouse Anti-Human CD33

Clonality

Monoclonal

Isotype

Mouse IgG1

Clone Number

WM53

Host

Mouse

Species Reactivity

Human

Form/Format

SpectralRed (SPRD) Conjugate

Specificity

Human CD33
CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.1-4

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Molecular Weight

39,825 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (51728335..51743274). Location: 19q13.3

OMIM

159590

3D Structure

ModBase 3D Structure for P20138

Characterization

To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-CD33 antibody

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot

Application Notes for anti-CD33 antibody

Flow Cytometry Suggested Dilution: 10 uL/10^6 cells

Product References and Citations for anti-CD33 antibody

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD33 Section, Academic Press, New York, p. 213.
4. Freeman, S.D., S. Kelm, E.K. Barber, and P.R. Crocker. 1995. Blood 85:2005.

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #

32187777

NCBI GeneID

945

UniProt Primary Accession #

P20138

UniProt Secondary Accession #

Q8TD24

UniProt Related Accession #

P20138; Q546G0; Q8NF11

NCBI Official Full Name

CD33 antigen (gp67)

NCBI Official Synonym Full Names

CD33 molecule

NCBI Official Symbol

CD33

NCBI Official Synonym Symbols

p67; SIGLEC3; FLJ00391; SIGLEC-3

UniProt Protein Name

Myeloid cell surface antigen CD33

UniProt Synonym Protein Names

Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67

Protein Family

Myeloid cell surface antigen

UniProt Gene Name

CD33

UniProt Synonym Gene Names

SIGLEC3

UniProt Entry Name

CD33_HUMAN

NCBI Protein Information

myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3

UniProt Comments for CD33

Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Ref.5 Ref.7

Subunit structure: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Ref.5 Ref.6

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Tissue specificity: Monocytic/myeloid lineage cells.

Domain: Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Post-translational modification: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Sequence similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Research Articles on CD33

1. Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA1 are associated with late-onset Alzheimer's disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD33 antibody

Reference Product	PubMed Publications
SRC antibody	>18 publications with CD33 and SRC
PTPN11 antibody	>11 publications with CD33 and PTPN11
PTPN6 antibody	>7 publications with CD33 and PTPN6

Pathways associated with anti-CD33 antibody

Products by Pathway	Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD33 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>29 publications with CD33 and Breast Neoplasms
Glioma Antibodies	>2 publications with CD33 and Glioma
Head and Neck Neoplasms Antibodies	>2 publications with CD33 and Head and Neck Neoplasms
Alzheimer Disease Antibodies	>2 publications with CD33 and Alzheimer Disease
Carcinoma, Squamous Cell Antibodies	>1 publications with CD33 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-CD33 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1187 publications with CD33 and Blood
Bone Antibodies	>863 publications with CD33 and Bone
Bone Marrow Antibodies	>852 publications with CD33 and Bone Marrow
Liver Antibodies	>74 publications with CD33 and Liver
Lymph Node Antibodies	>43 publications with CD33 and Lymph Node
Lung Antibodies	>36 publications with CD33 and Lung
Thymus Antibodies	>28 publications with CD33 and Thymus
Brain Antibodies	>16 publications with CD33 and Brain
Kidney Antibodies	>15 publications with CD33 and Kidney
Connective Tissue Antibodies	>8 publications with CD33 and Connective Tissue