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CD43 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674251

MBS674251 is an antibody targeting CD43. Also known as Mouse Anti-Human CD43-UNLB. Reactive in Human. Host: Mouse. More details

$225 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD43, Monoclonal Antibody
Full Product Name
Mouse Anti-Human CD43-UNLB
Product Synonym Names
Mouse Anti-Human CD43 - Purified, Unlabeled Antibody; Mouse Anti-Human CD43
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
DF-T1
Host
Mouse
Species Reactivity
Human
Form/Format
Purified (UNLB) Antibody
Specificity
Human CD43
CD43, a member of the sialomucin family of cell surface receptors, is a 95-135 kDa type I transmembrane glycoprotein whose variability in molecular weight is dependent upon the nature of the extracellular O-glycans. It is the major sialoglycoprotein on thymocytes, T cells and neutrophils. It is also present on activated B cells, plasma cells, NK cells, granulocytes, monocytes, macrophages, platelets and bone marrow hematopoietic stem cells. CD43, which binds to ICAM-1 (CD54), appears to function as an anti-adhesion molecule, inhibiting T cell interactions, including T cell killing, and by increasing the threshold for T cell activation.1-4
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-CD43 antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections)
Application Notes for anti-CD43 antibody
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-CD43 antibody

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD43 Section, Academic Press, New York, p. 238.
3. Cyster, J.G., D.M. Shotton, and A.F. Williams 1991. EMBO J. 10:893.
4. Manjunath, N., M. Correa, M. Ardman, and B. Ardman. 1995. Nature 377:535.

Other Notes

Small volumes of anti-CD43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD43. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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