• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Mullerian Inhibiting Substance/Mullerian Hormone (MIS/AMH) ELISA Kit for Porcine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS006757

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MIS/AMH concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine MIS/AMH antibody to coat Microelisa… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mullerian Inhibiting Substance/Mullerian Hormone (MIS/AMH), ELISA Kit
Full Product Name
Porcine Mullerian Inhibiting Substance/Mullerian Hormone ELISA Kit
Product Gene Name
MIS/AMH elisa kit
Species Reactivity
Porcine
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Detection Range
0.25ng/ml-8ng/ml.
Sensitivity
0.1ng/ml.
Specificity
No significant cross-reactivity or interference between Porcine MIS/AMH and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
59,195 Da
OMIM
261550
3D Structure
ModBase 3D Structure for P03971
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MIS/AMH concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine MIS/AMH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIS/AMH and MIS/AMH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIS/AMH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of MIS/AMH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIS/AMH purchase

MBS006757 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mullerian Inhibiting Substance/Mullerian Hormone (MIS/AMH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIS/AMH. The ELISA analytical biochemical technique of the MBS006757 kit is based on MIS/AMH antibody-MIS/AMH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIS/AMH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIS/AMH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MIS/AMH. It may not necessarily be applicable to this product.

NCBI GI #
157266298
NCBI GeneID
268
NCBI Accession #
NP_000470.2
UniProt Primary Accession #
P03971
UniProt Secondary Accession #
O75246; Q6GTN3
UniProt Related Accession #
P03971
GenBank Nucleotide #
NM_000479.3
NCBI Official Full Name
muellerian-inhibiting factor
NCBI Official Synonym Full Names
anti-Mullerian hormone
NCBI Official Symbol
AMH
NCBI Official Synonym Symbols
MIF; MIS
UniProt Protein Name
Muellerian-inhibiting factor
UniProt Synonym Protein Names
Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance
UniProt Gene Name
AMH
UniProt Synonym Gene Names
MIF; AMH; MIS
UniProt Entry Name
MIS_HUMAN
NCBI Protein Information
muellerian-inhibiting factor; anti-Muellerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance; muellerian-inhibiting substance

NCBI Summary for MIS/AMH

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for MIS/AMH

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; hormone activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: response to drug; sex determination; Mullerian duct regression; cell-cell signaling; gonadal mesoderm development; preantral ovarian follicle growth; sex differentiation; response to organic cyclic substance; urogenital system development; activation of NF-kappaB transcription factor; aging

Disease: Persistent Mullerian Duct Syndrome, Types I And Ii
Show more

Research Articles on MIS/AMH

1. Serum AMH was significantly lower 2 days and 3 months following hysterectomy compared with the pre-operative level. Hysterectomy may have a more lasting adverse effect on ovarian reserve than myomectomy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIS/AMH elisa kit

 Reference Product  PubMed Publications
 FSH elisa kit  >545 publications with MIS/AMH and FSH
 SOX9 elisa kit  >75 publications with MIS/AMH and SOX9
 AMHR2 elisa kit  >32 publications with MIS/AMH and AMHR2
 GATA4 elisa kit  >21 publications with MIS/AMH and GATA4
 KITLG elisa kit  >13 publications with MIS/AMH and KITLG
 ZFPM2 elisa kit  >3 publications with MIS/AMH and ZFPM2
 SMAD5 elisa kit  >3 publications with MIS/AMH and SMAD5
 SMAD9 elisa kit  >1 publications with MIS/AMH and SMAD9
 ACVR1 elisa kit  >1 publications with MIS/AMH and ACVR1

Pathways associated with MIS/AMH elisa kit

 Products by Pathway  Pathway Diagram
 ALK2 Signaling Events Pathway antibodies  ALK2 Signaling Events Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram

Diseases associated with MIS/AMH elisa kit

 Disease Name  Pubmed Publications
 Female Urogenital Diseases Antibodies  >549 publications with MIS/AMH and Female Urogenital Diseases
 Gonadal Disorders Antibodies  >449 publications with MIS/AMH and Gonadal Disorders
 Neoplasms Antibodies  >309 publications with MIS/AMH and Neoplasms
 Ovarian Diseases Antibodies  >306 publications with MIS/AMH and Ovarian Diseases
 Infertility, Female Antibodies  >264 publications with MIS/AMH and Infertility, Female
 Urogenital Abnormalities Antibodies  >151 publications with MIS/AMH and Urogenital Abnormalities
 Genital Diseases, Male Antibodies  >129 publications with MIS/AMH and Genital Diseases, Male
 Disorders of Sex Development Antibodies  >129 publications with MIS/AMH and Disorders of Sex Development
 Infertility, Male Antibodies  >76 publications with MIS/AMH and Infertility, Male
 Testicular Diseases Antibodies  >65 publications with MIS/AMH and Testicular Diseases

Organs/Tissues associated with MIS/AMH elisa kit

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >319 publications with MIS/AMH and Testis
 Uterus Antibodies  >66 publications with MIS/AMH and Uterus
 Brain Antibodies  >50 publications with MIS/AMH and Brain
 Embryonic Tissue Antibodies  >47 publications with MIS/AMH and Embryonic Tissue
 Lung Antibodies  >19 publications with MIS/AMH and Lung
 Prostate Antibodies  >12 publications with MIS/AMH and Prostate
 Intestine Antibodies  >9 publications with MIS/AMH and Intestine
 Eye Antibodies  >4 publications with MIS/AMH and Eye
 Stomach Antibodies  >2 publications with MIS/AMH and Stomach
 Mammary Gland Antibodies  >2 publications with MIS/AMH and Mammary Gland
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000